BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Atropa_belladonna.fasta 67,848 sequences; 17,323,337 total letters Query= Length=134 Score E Sequences producing significant alignments: (Bits) Value aba_locus_126286_iso_1_len_493_ver_2 266 7e-92 aba_locus_126285_iso_1_len_446_ver_2 266 7e-92 aba_locus_11545_iso_2_len_1291_ver_2 28.1 2.5 aba_locus_11545_iso_5_len_1457_ver_2 28.1 2.5 aba_locus_11545_iso_7_len_1483_ver_2 28.1 2.5 aba_locus_11545_iso_4_len_1483_ver_2 28.1 2.6 aba_locus_10442_iso_1_len_1226_ver_2 27.7 3.6 aba_locus_11545_iso_3_len_1839_ver_2 27.7 3.8 > Query1 on aba_locus_126286_iso_1_len_493_ver_2 Length=134 Score = 266 bits (679), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 134/134 (100%), Positives = 134/134 (100%), Gaps = 0/134 (0%) Query 1 MDLVAKYQGVVGRVFGNENSGSSEDSYVERLLDRISNGVLAEDRRAAMLELQSIVSESRA 60 MDLVAKYQGVVGRVFGNENSGSSEDSYVERLLDRISNGVLAEDRRAAMLELQSIVSESRA Sbjct 1 MDLVAKYQGVVGRVFGNENSGSSEDSYVERLLDRISNGVLAEDRRAAMLELQSIVSESRA 60 Query 61 GQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTLMNSDL 120 GQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTLMNSDL Sbjct 61 GQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTLMNSDL 120 Query 121 LSREVDNISLLLSL 134 LSREVDNISLLLSL Sbjct 121 LSREVDNISLLLSL 134 > Query1 on aba_locus_126285_iso_1_len_446_ver_2 Length=134 Score = 266 bits (679), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 134/134 (100%), Positives = 134/134 (100%), Gaps = 0/134 (0%) Query 1 MDLVAKYQGVVGRVFGNENSGSSEDSYVERLLDRISNGVLAEDRRAAMLELQSIVSESRA 60 MDLVAKYQGVVGRVFGNENSGSSEDSYVERLLDRISNGVLAEDRRAAMLELQSIVSESRA Sbjct 1 MDLVAKYQGVVGRVFGNENSGSSEDSYVERLLDRISNGVLAEDRRAAMLELQSIVSESRA 60 Query 61 GQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTLMNSDL 120 GQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTLMNSDL Sbjct 61 GQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTLMNSDL 120 Query 121 LSREVDNISLLLSL 134 LSREVDNISLLLSL Sbjct 121 LSREVDNISLLLSL 134 > Query1 on aba_locus_11545_iso_2_len_1291_ver_2 Length=390 Score = 28.1 bits (61), Expect = 2.5, Method: Compositional matrix adjust. Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (7%) Query 51 LQSIVSESRAGQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHA---KGP 107 L + E R ++ F FP + + L+++ DD E + L +++ LT + K Sbjct 245 LSKAIKEVRNDKVQFIWTQFPELYAYLRKQADDSEKLNKKLAEMIALLTCEKQSNSRKSI 304 Query 108 ANEVQPTLMNSDLLSR 123 +V P + D+LSR Sbjct 305 KFDVTPDM--KDILSR 318 > Query1 on aba_locus_11545_iso_5_len_1457_ver_2 Length=390 Score = 28.1 bits (61), Expect = 2.5, Method: Compositional matrix adjust. Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (7%) Query 51 LQSIVSESRAGQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHA---KGP 107 L + E R ++ F FP + + L+++ DD E + L +++ LT + K Sbjct 245 LSKAIKEVRNDKVQFIWTQFPELYAYLRKQADDSEKLNKKLAEMIALLTCEKQSNSRKSI 304 Query 108 ANEVQPTLMNSDLLSR 123 +V P + D+LSR Sbjct 305 KFDVTPDM--KDILSR 318 > Query1 on aba_locus_11545_iso_7_len_1483_ver_2 Length=390 Score = 28.1 bits (61), Expect = 2.5, Method: Compositional matrix adjust. Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (7%) Query 51 LQSIVSESRAGQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHA---KGP 107 L + E R ++ F FP + + L+++ DD E + L +++ LT + K Sbjct 245 LSKAIKEVRNDKVQFIWTQFPELYAYLRKQADDSEKLNKKLAEMIALLTCEKQSNSRKSI 304 Query 108 ANEVQPTLMNSDLLSR 123 +V P + D+LSR Sbjct 305 KFDVTPDM--KDILSR 318 > Query1 on aba_locus_11545_iso_4_len_1483_ver_2 Length=390 Score = 28.1 bits (61), Expect = 2.6, Method: Compositional matrix adjust. Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (7%) Query 51 LQSIVSESRAGQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHA---KGP 107 L + E R ++ F FP + + L+++ DD E + L +++ LT + K Sbjct 245 LSKAIKEVRNDKVQFIWTQFPELYAYLRKQADDSEKLNKKLAEMIALLTCEKQSNSRKSI 304 Query 108 ANEVQPTLMNSDLLSR 123 +V P + D+LSR Sbjct 305 KFDVTPDM--KDILSR 318 > Query1 on aba_locus_10442_iso_1_len_1226_ver_2 Length=298 Score = 27.7 bits (60), Expect = 3.6, Method: Compositional matrix adjust. Identities = 29/82 (35%), Positives = 43/82 (52%), Gaps = 15/82 (18%) Query 56 SESRAGQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHAKGPANEVQPTL 115 SESR ++ A +PVILS L+EE +E G+ E L + TPI EV Sbjct 224 SESRVYKVVSSAENYPVILSKLQEEGIKLEPDNGS-ELLPT--TPI--------EVDDEA 272 Query 116 M--NSDLLSR--EVDNISLLLS 133 M N DL+S+ E+D++ + + Sbjct 273 MELNKDLMSKLLELDDVDAVYT 294 > Query1 on aba_locus_11545_iso_3_len_1839_ver_2 Length=508 Score = 27.7 bits (60), Expect = 3.8, Method: Compositional matrix adjust. Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (7%) Query 51 LQSIVSESRAGQMAFGAMGFPVILSLLKEERDDVEMVRGALETLVSALTPIDHA---KGP 107 L + E R ++ F FP + + L+++ DD E + L +++ LT + K Sbjct 363 LSKAIKEVRNDKVQFIWTQFPELYAYLRKQADDSEKLNKKLAEMIALLTCEKQSNSRKSI 422 Query 108 ANEVQPTLMNSDLLSR 123 +V P + D+LSR Sbjct 423 KFDVTPDM--KDILSR 436 Lambda K H a alpha 0.315 0.132 0.349 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 507818925 Database: Atropa_belladonna.fasta Posted date: Mar 24, 2019 8:20 PM Number of letters in database: 17,323,337 Number of sequences in database: 67,848 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40