BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Rauvolfia_serpentina.fasta 85,221 sequences; 26,378,518 total letters Query= Length=141 Score E Sequences producing significant alignments: (Bits) Value rsa_locus_2917_iso_5_len_889_ver_2 283 3e-98 rsa_locus_2917_iso_4_len_1111_ver_2 202 6e-66 rsa_locus_2917_iso_3_len_1396_ver_2 205 2e-65 rsa_locus_2917_iso_8_len_1447_ver_2 204 6e-65 rsa_locus_2917_iso_2_len_1333_ver_2 202 1e-63 rsa_locus_41026_iso_1_len_626_ver_2 174 9e-55 rsa_locus_2917_iso_6_len_1544_ver_2 152 6e-45 rsa_locus_20757_iso_1_len_1537_ver_2 29.3 2.0 rsa_locus_20757_iso_2_len_1516_ver_2 29.3 2.0 rsa_locus_7249_iso_2_len_644_ver_2 27.7 4.2 > Query1 on rsa_locus_2917_iso_5_len_889_ver_2 Length=141 Score = 283 bits (724), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 141/141 (100%), Positives = 141/141 (100%), Gaps = 0/141 (0%) Query 1 EPHYEDLVAIFFXESQFHYCQDCLYYLLVLSPKAHCRYLVTTFKVVPRGTEGAVSVEGTL 60 EPHYEDLVAIFFXESQFHYCQDCLYYLLVLSPKAHCRYLVTTFKVVPRGTEGAVSVEGTL Sbjct 1 EPHYEDLVAIFFXESQFHYCQDCLYYLLVLSPKAHCRYLVTTFKVVPRGTEGAVSVEGTL 60 Query 61 AGLLASVLLASVGCLMGEINVTEAIICVIASQIANLGESVIGAALQEKEGFRWLNNDIVN 120 AGLLASVLLASVGCLMGEINVTEAIICVIASQIANLGESVIGAALQEKEGFRWLNNDIVN Sbjct 61 AGLLASVLLASVGCLMGEINVTEAIICVIASQIANLGESVIGAALQEKEGFRWLNNDIVN 120 Query 121 IINISIGSILAILMQQIIFQS 141 IINISIGSILAILMQQIIFQS Sbjct 121 IINISIGSILAILMQQIIFQS 141 > Query1 on rsa_locus_2917_iso_4_len_1111_ver_2 Length=181 Score = 202 bits (514), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 106/111 (95%), Positives = 108/111 (97%), Gaps = 2/111 (2%) Query 33 KAHCR--YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 90 KA+ + YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA Sbjct 71 KAYGKTTYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 130 Query 91 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS 141 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS Sbjct 131 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS 181 > Query1 on rsa_locus_2917_iso_3_len_1396_ver_2 Length=308 Score = 205 bits (522), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 104/105 (99%), Positives = 105/105 (100%), Gaps = 0/105 (0%) Query 37 RYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIASQIANL 96 +YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIASQIANL Sbjct 204 KYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIASQIANL 263 Query 97 GESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS 141 GESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS Sbjct 264 GESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS 308 > Query1 on rsa_locus_2917_iso_8_len_1447_ver_2 Length=325 Score = 204 bits (520), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 106/111 (95%), Positives = 108/111 (97%), Gaps = 2/111 (2%) Query 33 KAHCR--YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 90 KA+ + YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA Sbjct 215 KAYGKTTYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 274 Query 91 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS 141 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS Sbjct 275 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQS 325 > Query1 on rsa_locus_2917_iso_2_len_1333_ver_2 Length=362 Score = 202 bits (514), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 105/110 (95%), Positives = 107/110 (97%), Gaps = 2/110 (2%) Query 33 KAHCR--YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 90 KA+ + YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA Sbjct 215 KAYGKTTYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 274 Query 91 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQ 140 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQ Sbjct 275 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQ 324 > Query1 on rsa_locus_41026_iso_1_len_626_ver_2 Length=184 Score = 174 bits (440), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 85/110 (77%), Positives = 99/110 (90%), Gaps = 2/110 (2%) Query 33 KAHCR--YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 90 KA+ R YLVTTFKVVPRGTEGA+S+EGT AGL+AS+LLA V CLMGEI V EAIIC++A Sbjct 74 KAYGRTTYLVTTFKVVPRGTEGAISLEGTFAGLVASILLAFVACLMGEIKVPEAIICIVA 133 Query 91 SQIANLGESVIGAALQEKEGFRWLNNDIVNIINISIGSILAILMQQIIFQ 140 SQIANLGES+IGAA QEKEGFRWLNND VN+INI++GS+LA+LMQQII + Sbjct 134 SQIANLGESMIGAAFQEKEGFRWLNNDAVNVINITMGSVLAVLMQQIILK 183 > Query1 on rsa_locus_2917_iso_6_len_1544_ver_2 Length=297 Score = 152 bits (383), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 78/83 (94%), Positives = 80/83 (96%), Gaps = 2/83 (2%) Query 33 KAHCR--YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 90 KA+ + YLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA Sbjct 215 KAYGKTTYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEAIICVIA 274 Query 91 SQIANLGESVIGAALQEKEGFRW 113 SQIANLGESVIGAALQEKEGFRW Sbjct 275 SQIANLGESVIGAALQEKEGFRW 297 > Query1 on rsa_locus_20757_iso_1_len_1537_ver_2 Length=513 Score = 29.3 bits (64), Expect = 2.0, Method: Composition-based stats. Identities = 20/49 (41%), Positives = 28/49 (57%), Gaps = 3/49 (6%) Query 20 CQDCLYYLLVLSPKAHCRYL--VTTFKVVPRGTEGAV-SVEGTLAGLLA 65 C+D LY LLVL + R+L V KV+ +G +V S +L GLL+ Sbjct 138 CEDALYSLLVLGARRPIRHLASVAVAKVILKGDSISVYSRVSSLQGLLS 186 > Query1 on rsa_locus_20757_iso_2_len_1516_ver_2 Length=506 Score = 29.3 bits (64), Expect = 2.0, Method: Composition-based stats. Identities = 20/49 (41%), Positives = 28/49 (57%), Gaps = 3/49 (6%) Query 20 CQDCLYYLLVLSPKAHCRYL--VTTFKVVPRGTEGAV-SVEGTLAGLLA 65 C+D LY LLVL + R+L V KV+ +G +V S +L GLL+ Sbjct 138 CEDALYSLLVLGARRPIRHLASVAVAKVILKGDSISVYSRVSSLQGLLS 186 > Query1 on rsa_locus_7249_iso_2_len_644_ver_2 Length=140 Score = 27.7 bits (60), Expect = 4.2, Method: Compositional matrix adjust. Identities = 16/60 (27%), Positives = 29/60 (48%), Gaps = 0/60 (0%) Query 25 YYLLVLSPKAHCRYLVTTFKVVPRGTEGAVSVEGTLAGLLASVLLASVGCLMGEINVTEA 84 Y++L SP+ R+ K+ +G +S+ T+ G+ +L + G L EI +A Sbjct 27 YHILHHSPELVFRFYQDISKLGRPEDDGTMSMTSTMQGINEKILSLNYGDLSAEIKSVDA 86 Lambda K H a alpha 0.325 0.140 0.410 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 885742320 Database: Rauvolfia_serpentina.fasta Posted date: Mar 24, 2019 9:10 PM Number of letters in database: 26,378,518 Number of sequences in database: 85,221 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40