BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Panax_quinquefolius.fasta 79,847 sequences; 23,540,582 total letters Query= Length=168 Score E Sequences producing significant alignments: (Bits) Value pqa_locus_3641_iso_4_len_996_ver_2 347 8e-123 pqa_locus_3641_iso_1_len_1089_ver_2 338 4e-119 pqa_locus_3641_iso_7_len_1100_ver_2 338 4e-119 pqa_locus_3641_iso_6_len_1103_ver_2 316 2e-110 pqa_locus_3641_iso_2_len_1230_ver_2 89.4 2e-22 pqa_locus_3641_iso_3_len_1230_ver_2 89.0 2e-22 pqa_locus_4_iso_5489_len_351_ver_2 32.0 0.15 pqa_locus_4_iso_274_len_351_ver_2 32.0 0.15 pqa_locus_39161_iso_1_len_337_ver_2 26.6 4.3 > Query1 on pqa_locus_3641_iso_4_len_996_ver_2 Length=168 Score = 347 bits (891), Expect = 8e-123, Method: Compositional matrix adjust. Identities = 168/168 (100%), Positives = 168/168 (100%), Gaps = 0/168 (0%) Query 1 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA 60 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA Sbjct 1 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA 60 Query 61 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI 120 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI Sbjct 61 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI 120 Query 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA Sbjct 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 > Query1 on pqa_locus_3641_iso_1_len_1089_ver_2 Length=170 Score = 338 bits (867), Expect = 4e-119, Method: Compositional matrix adjust. Identities = 163/168 (97%), Positives = 164/168 (98%), Gaps = 0/168 (0%) Query 1 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA 60 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA Sbjct 1 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA 60 Query 61 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI 120 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI Sbjct 61 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI 120 Query 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKS SPE+SP KAA Sbjct 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSGASPESSPAEKAA 168 > Query1 on pqa_locus_3641_iso_7_len_1100_ver_2 Length=170 Score = 338 bits (867), Expect = 4e-119, Method: Compositional matrix adjust. Identities = 163/168 (97%), Positives = 164/168 (98%), Gaps = 0/168 (0%) Query 1 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA 60 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA Sbjct 1 MLRRTTGRILGLGLRQEMPAAMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVGA 60 Query 61 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI 120 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI Sbjct 61 PACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTEI 120 Query 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKS SPE+SP KAA Sbjct 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSGASPESSPAEKAA 168 > Query1 on pqa_locus_3641_iso_6_len_1103_ver_2 Length=171 Score = 316 bits (810), Expect = 2e-110, Method: Compositional matrix adjust. Identities = 155/169 (92%), Positives = 159/169 (94%), Gaps = 1/169 (1%) Query 1 MLRRTTGRILGLGLRQEMPA-AMQVVPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVG 59 MLR TT RILG GLRQEM A A Q++PRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVG Sbjct 1 MLRHTTRRILGSGLRQEMLAVATQIMPRFYHERVVDHYNNPRNVGSFDKSDPTVGTGLVG 60 Query 60 APACGDVMKLQIKVDDKTGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTE 119 APACGDVMKLQIKVDDK+GKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTE Sbjct 61 APACGDVMKLQIKVDDKSGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVSIKNTE 120 Query 120 IAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 IAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKS SPE+SP KAA Sbjct 121 IAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSGASPESSPAEKAA 169 > Query1 on pqa_locus_3641_iso_2_len_1230_ver_2 Length=104 Score = 89.4 bits (220), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 45/50 (90%), Positives = 46/50 (92%), Gaps = 0/50 (0%) Query 119 EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKS SPE+SP KAA Sbjct 53 EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSGASPESSPAEKAA 102 > Query1 on pqa_locus_3641_iso_3_len_1230_ver_2 Length=104 Score = 89.0 bits (219), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 45/50 (90%), Positives = 46/50 (92%), Gaps = 0/50 (0%) Query 119 EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSEVSPEASPVVKAA 168 EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKS SPE+SP KAA Sbjct 53 EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKSGASPESSPAEKAA 102 > Query1 on pqa_locus_4_iso_5489_len_351_ver_2 Length=118 Score = 32.0 bits (71), Expect = 0.15, Method: Compositional matrix adjust. Identities = 23/73 (32%), Positives = 32/73 (44%), Gaps = 12/73 (16%) Query 63 CGDVMKLQIKVDDKTGKIVDACFKT------FGCGSAIASSSVATEWV------KGKQME 110 CG +MKLQ K D K+V F G G+ + + A WV K + E Sbjct 16 CGALMKLQPKGMDMAAKLVKKAFSVCDSQPATGRGNKVVKTGPAPGWVQQPLITKRENTE 75 Query 111 EVVSIKNTEIAKH 123 E + K T++ KH Sbjct 76 EYLERKTTDLDKH 88 > Query1 on pqa_locus_4_iso_274_len_351_ver_2 Length=118 Score = 32.0 bits (71), Expect = 0.15, Method: Compositional matrix adjust. Identities = 23/73 (32%), Positives = 32/73 (44%), Gaps = 12/73 (16%) Query 63 CGDVMKLQIKVDDKTGKIVDACFKT------FGCGSAIASSSVATEWV------KGKQME 110 CG +MKLQ K D K+V F G G+ + + A WV K + E Sbjct 16 CGALMKLQPKGMDMAAKLVKKAFSVCDSQPATGRGNKVVKTGPAPGWVQQPLITKRENTE 75 Query 111 EVVSIKNTEIAKH 123 E + K T++ KH Sbjct 76 EYLERKTTDLDKH 88 > Query1 on pqa_locus_39161_iso_1_len_337_ver_2 Length=38 Score = 26.6 bits (57), Expect = 4.3, Method: Composition-based stats. Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 2/30 (7%) Query 46 FDKSDPTVGTGLVGA-PACGDVMKLQIKVD 74 FD SDP V TGLVG ACG + + ++ D Sbjct 3 FD-SDPFVQTGLVGMYAACGHIQEARLVFD 31 Lambda K H a alpha 0.316 0.131 0.378 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 1164723541 Database: Panax_quinquefolius.fasta Posted date: Mar 24, 2019 8:59 PM Number of letters in database: 23,540,582 Number of sequences in database: 79,847 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40