BLAST Results




BLASTP 2.2.28+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.


Reference for
composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: Hoodia_gordonii.fasta
           41,349 sequences; 7,589,719 total letters



Query= 
Length=91
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  hga_locus_393_iso_1_len_499_ver_2                                    183    5e-61
  hga_locus_16504_iso_7_len_509_ver_2                                 25.8    2.1  
  hga_locus_16504_iso_6_len_560_ver_2                                 25.8    2.5  
  hga_locus_16504_iso_1_len_279_ver_2                                 25.0    3.9  
  hga_locus_16504_iso_2_len_454_ver_2                                 24.3    4.3  


> Query1 on hga_locus_393_iso_1_len_499_ver_2
Length=91

 Score =  183 bits (465),  Expect = 5e-61, Method: Compositional matrix adjust.
 Identities = 90/90 (100%), Positives = 90/90 (100%), Gaps = 0/90 (0%)

Query  2   RSSDLSAVGAFYELLSQSSLNVLHPDDKNPVAPVELCPILKTLYKILITREQEVKGILQA  61
           RSSDLSAVGAFYELLSQSSLNVLHPDDKNPVAPVELCPILKTLYKILITREQEVKGILQA
Sbjct  2   RSSDLSAVGAFYELLSQSSLNVLHPDDKNPVAPVELCPILKTLYKILITREQEVKGILQA  61

Query  62  LRDENLNDPRDRIEIAQSHAFYRPSLLGQP  91
           LRDENLNDPRDRIEIAQSHAFYRPSLLGQP
Sbjct  62  LRDENLNDPRDRIEIAQSHAFYRPSLLGQP  91


> Query1 on hga_locus_16504_iso_7_len_509_ver_2
Length=117

 Score = 25.8 bits (55),  Expect = 2.1, Method: Compositional matrix adjust.
 Identities = 11/25 (44%), Positives = 14/25 (56%), Gaps = 0/25 (0%)

Query  49   ITREQEVKGILQALRDENLNDPRDR  73
            I R Q +K I   ++  NL DP DR
Sbjct  84   IPRTQALKKIWAYIKQNNLQDPEDR  108


> Query1 on hga_locus_16504_iso_6_len_560_ver_2
Length=188

 Score = 25.8 bits (55),  Expect = 2.5, Method: Compositional matrix adjust.
 Identities = 11/25 (44%), Positives = 14/25 (56%), Gaps = 0/25 (0%)

Query  49   ITREQEVKGILQALRDENLNDPRDR  73
            I R Q +K I   ++  NL DP DR
Sbjct  155  IPRTQALKKIWAYIKQNNLQDPEDR  179


> Query1 on hga_locus_16504_iso_1_len_279_ver_2
Length=94

 Score = 25.0 bits (53),  Expect = 3.9, Method: Compositional matrix adjust.
 Identities = 11/25 (44%), Positives = 14/25 (56%), Gaps = 0/25 (0%)

Query  49  ITREQEVKGILQALRDENLNDPRDR  73
           I R Q +K I   ++  NL DP DR
Sbjct  61  IPRTQALKKIWAYIKQNNLQDPEDR  85


> Query1 on hga_locus_16504_iso_2_len_454_ver_2
Length=68

 Score = 24.3 bits (51),  Expect = 4.3, Method: Compositional matrix adjust.
 Identities = 11/25 (44%), Positives = 14/25 (56%), Gaps = 0/25 (0%)

Query  49  ITREQEVKGILQALRDENLNDPRDR  73
           I R Q +K I   ++  NL DP DR
Sbjct  35  IPRTQALKKIWAYIKQNNLQDPEDR  59



Lambda      K        H        a         alpha
   0.317    0.136    0.385    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 191358864


  Database: Hoodia_gordonii.fasta
    Posted date:  Mar 24, 2019  8:39 PM
  Number of letters in database: 7,589,719
  Number of sequences in database:  41,349



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40