BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Digitalis_purpurea.fasta 92,122 sequences; 30,569,573 total letters Query= Length=256 Score E Sequences producing significant alignments: (Bits) Value dpa_locus_4517_iso_2_len_895_ver_4 522 0.0 dpa_locus_4517_iso_8_len_925_ver_4 522 0.0 dpa_locus_4517_iso_3_len_925_ver_4 478 3e-171 dpa_locus_4517_iso_7_len_925_ver_4 455 2e-162 dpa_locus_4517_iso_6_len_888_ver_4 354 8e-123 dpa_locus_4517_iso_1_len_818_ver_4 311 3e-107 dpa_locus_4517_iso_4_len_959_ver_4 263 2e-87 dpa_locus_7314_iso_1_len_787_ver_4 114 6e-30 dpa_locus_7314_iso_2_len_807_ver_4 114 6e-30 dpa_locus_18986_iso_1_len_689_ver_4 28.9 4.6 > Query1 on dpa_locus_4517_iso_2_len_895_ver_4 Length=256 Score = 522 bits (1344), Expect = 0.0, Method: Compositional matrix adjust. Identities = 256/256 (100%), Positives = 256/256 (100%), Gaps = 0/256 (0%) Query 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA Sbjct 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 Query 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP Sbjct 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 Query 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY Sbjct 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 Query 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL Sbjct 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 Query 241 LVTMLTTLDLLVRMNT 256 LVTMLTTLDLLVRMNT Sbjct 241 LVTMLTTLDLLVRMNT 256 > Query1 on dpa_locus_4517_iso_8_len_925_ver_4 Length=256 Score = 522 bits (1344), Expect = 0.0, Method: Compositional matrix adjust. Identities = 256/256 (100%), Positives = 256/256 (100%), Gaps = 0/256 (0%) Query 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA Sbjct 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 Query 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP Sbjct 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 Query 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY Sbjct 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 Query 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL Sbjct 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 Query 241 LVTMLTTLDLLVRMNT 256 LVTMLTTLDLLVRMNT Sbjct 241 LVTMLTTLDLLVRMNT 256 > Query1 on dpa_locus_4517_iso_3_len_925_ver_4 Length=256 Score = 478 bits (1229), Expect = 3e-171, Method: Compositional matrix adjust. Identities = 250/256 (98%), Positives = 252/256 (98%), Gaps = 0/256 (0%) Query 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA Sbjct 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 Query 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 SANFSIP LSPLFGNACQENVCG+GNCV SNNS FGF+CEC PGWKQARSDDDDDLKFLP Sbjct 61 SANFSIPFLSPLFGNACQENVCGRGNCVASNNSAFGFQCECLPGWKQARSDDDDDLKFLP 120 Query 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY Sbjct 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 Query 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL Sbjct 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 Query 241 LVTMLTTLDLLVRMNT 256 LVTMLTTLDLLVRMNT Sbjct 241 LVTMLTTLDLLVRMNT 256 > Query1 on dpa_locus_4517_iso_7_len_925_ver_4 Length=256 Score = 455 bits (1171), Expect = 2e-162, Method: Compositional matrix adjust. Identities = 240/256 (94%), Positives = 242/256 (95%), Gaps = 0/256 (0%) Query 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA Sbjct 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 Query 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP Sbjct 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 Query 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY Sbjct 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGY 180 Query 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRLIPSVEVAL 240 YNLFNTTAFPCYHQCAFGVDCADLGLNF NTT S +GSSPSSANDSNS+ RLIP VE Sbjct 181 YNLFNTTAFPCYHQCAFGVDCADLGLNFTNTTASRKGSSPSSANDSNSEMRLIPGVEFTW 240 Query 241 LVTMLTTLDLLVRMNT 256 L TMLT L L V NT Sbjct 241 LATMLTALALFVWKNT 256 > Query1 on dpa_locus_4517_iso_6_len_888_ver_4 Length=244 Score = 354 bits (909), Expect = 8e-123, Method: Compositional matrix adjust. Identities = 194/222 (87%), Positives = 200/222 (90%), Gaps = 5/222 (2%) Query 35 FLNMATVHYAPFLTITLIIVFQSYLASANFSIPLLSPLFGNACQENVCGKGNCVTSNNSN 94 F ++ A FLT + F + A SI ++ PL GNACQENVCGKGNCVTSNNSN Sbjct 28 FDDLLITGVALFLTYS----FVGWSEIAKISI-IVFPLIGNACQENVCGKGNCVTSNNSN 82 Query 95 FGFECECQPGWKQARSDDDDDLKFLPCVVPNCTLNYTCENAAPPAPANPNRANLSFFDPC 154 FGFECECQPGWKQARSDDDDDLKFLPCVVPNCTLNYTCENAAPPAPANPNRANLSFFDPC Sbjct 83 FGFECECQPGWKQARSDDDDDLKFLPCVVPNCTLNYTCENAAPPAPANPNRANLSFFDPC 142 Query 155 FWTDCGGGTCNKTSLFTHTCLCEEGYYNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTS 214 FWTDCGGGTCNKTSLFTHTCLCEEGYYNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTS Sbjct 143 FWTDCGGGTCNKTSLFTHTCLCEEGYYNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTS 202 Query 215 GQGSSPSSANDSNSKTRLIPSVEVALLVTMLTTLDLLVRMNT 256 GQGSSPSSANDSNSKTRLIPSVEVALLVTMLTTLDLLVRMNT Sbjct 203 GQGSSPSSANDSNSKTRLIPSVEVALLVTMLTTLDLLVRMNT 244 > Query1 on dpa_locus_4517_iso_1_len_818_ver_4 Length=154 Score = 311 bits (797), Expect = 3e-107, Method: Compositional matrix adjust. Identities = 152/152 (100%), Positives = 152/152 (100%), Gaps = 0/152 (0%) Query 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA Sbjct 1 ILIHFTPLLKNTIEYLTFILALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLA 60 Query 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP Sbjct 61 SANFSIPLLSPLFGNACQENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDLKFLP 120 Query 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFD 152 CVVPNCTLNYTCENAAPPAPANPNRANLSFFD Sbjct 121 CVVPNCTLNYTCENAAPPAPANPNRANLSFFD 152 > Query1 on dpa_locus_4517_iso_4_len_959_ver_4 Length=221 Score = 263 bits (672), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 144/144 (100%), Positives = 144/144 (100%), Gaps = 0/144 (0%) Query 113 DDDLKFLPCVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTH 172 DDDLKFLPCVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTH Sbjct 78 DDDLKFLPCVVPNCTLNYTCENAAPPAPANPNRANLSFFDPCFWTDCGGGTCNKTSLFTH 137 Query 173 TCLCEEGYYNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRL 232 TCLCEEGYYNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRL Sbjct 138 TCLCEEGYYNLFNTTAFPCYHQCAFGVDCADLGLNFMNTTTSGQGSSPSSANDSNSKTRL 197 Query 233 IPSVEVALLVTMLTTLDLLVRMNT 256 IPSVEVALLVTMLTTLDLLVRMNT Sbjct 198 IPSVEVALLVTMLTTLDLLVRMNT 221 Score = 44.7 bits (104), Expect = 5e-05, Method: Compositional matrix adjust. Identities = 21/24 (88%), Positives = 21/24 (88%), Gaps = 0/24 (0%) Query 38 MATVHYAPFLTITLIIVFQSYLAS 61 M TVHY PFL ITLIIVFQSYLAS Sbjct 1 MVTVHYPPFLKITLIIVFQSYLAS 24 > Query1 on dpa_locus_7314_iso_1_len_787_ver_4 Length=214 Score = 114 bits (286), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 68/138 (49%), Positives = 89/138 (64%), Gaps = 5/138 (4%) Query 75 NACQENVCGKGNCVTSNNSNFGFECECQPGWKQAR---SDDDDDLKFLPCVVPNCTLNYT 131 N C E CG+G+C S + F F C+C+ GW++ R DD+D L+FLPC++PNC+L+Y+ Sbjct 36 NTCNEVQCGRGSCEASTDYLFNFRCDCEDGWRRTRLDDDDDEDHLQFLPCIIPNCSLDYS 95 Query 132 CENAAPPAPANPNRA-NLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGYYNLFNTTAFP 190 C AAPPAPA P+ + S FDPC W CG GTC + +HTC C GY NL N +AFP Sbjct 96 CMPAAPPAPAPPSLPYDKSVFDPCKWIYCGEGTCTLNTTRSHTCQCRPGYSNLLNISAFP 155 Query 191 CYHQCAFGV-DCADLGLN 207 CY+ CA G C LG+ Sbjct 156 CYNDCAVGSPSCERLGIR 173 > Query1 on dpa_locus_7314_iso_2_len_807_ver_4 Length=214 Score = 114 bits (286), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 68/138 (49%), Positives = 89/138 (64%), Gaps = 5/138 (4%) Query 75 NACQENVCGKGNCVTSNNSNFGFECECQPGWKQAR---SDDDDDLKFLPCVVPNCTLNYT 131 N C E CG+G+C S + F F C+C+ GW++ R DD+D L+FLPC++PNC+L+Y+ Sbjct 36 NTCNEVQCGRGSCEASTDYLFNFRCDCEDGWRRTRLDDDDDEDHLQFLPCIIPNCSLDYS 95 Query 132 CENAAPPAPANPNRA-NLSFFDPCFWTDCGGGTCNKTSLFTHTCLCEEGYYNLFNTTAFP 190 C AAPPAPA P+ + S FDPC W CG GTC + +HTC C GY NL N +AFP Sbjct 96 CMPAAPPAPAPPSLPYDKSVFDPCKWIYCGEGTCTLNTTRSHTCQCRPGYSNLLNISAFP 155 Query 191 CYHQCAFGV-DCADLGLN 207 CY+ CA G C LG+ Sbjct 156 CYNDCAVGSPSCERLGIR 173 > Query1 on dpa_locus_18986_iso_1_len_689_ver_4 Length=159 Score = 28.9 bits (63), Expect = 4.6, Method: Compositional matrix adjust. Identities = 24/99 (24%), Positives = 38/99 (38%), Gaps = 2/99 (2%) Query 20 LALFHTFHSTPASLCFLNMATVHYAPFLTITLIIVFQSYLASANFSIPLLSPLFG--NAC 77 L +F F + V P+L ++ Y N IP L + G NA Sbjct 29 LEIFDDSSYKEKGREFCDGLGVFDFPWLKEGVVYNGDEYFDHDNTFIPCLEEVSGTSNAN 88 Query 78 QENVCGKGNCVTSNNSNFGFECECQPGWKQARSDDDDDL 116 + C + + V+++ FGF P + S +DDD Sbjct 89 LDQSCDEESLVSNHEEKFGFAANLDPSCEDKSSFEDDDF 127 Lambda K H a alpha 0.324 0.136 0.446 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3225394071 Database: Digitalis_purpurea.fasta Posted date: Mar 24, 2019 8:30 PM Number of letters in database: 30,569,573 Number of sequences in database: 92,122 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40