BLAST Results
TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Dioscorea_rotundata.fa 21 sequences; 456,674,974 total letters Query= Dr00002 AED:0.45 eAED:0.45 QI:231|1|0.66|1|1|1|3|0|749 Glucose-methanol-choline oxidoreductase, N-terminal|Glucose-methanol-choline oxidoreductase, C-terminal|Alcohol dehydrogenase, long-chain Length=749 Score E Sequences producing significant alignments: (Bits) Value BDMI01000011.1 Dioscorea rotundata DNA, contig: Chromosome_11_P... 1182 0.0 BDMI01000002.1 Dioscorea rotundata DNA, contig: Chromosome_02_P... 629 0.0 BDMI01000004.1 Dioscorea rotundata DNA, contig: Chromosome_04_P... 88.2 4e-24 > Dr00002 on BDMI01000011.1 Dioscorea rotundata DNA, contig: Chromosome_11_P1_11_P2_9, cultivar: TDr96_F1, whole genome shotgun sequence Length=17195437 Score = 1182 bits (3059), Expect = 0.0, Method: Compositional matrix adjust. Identities = 582/582 (100%), Positives = 582/582 (100%), Gaps = 0/582 (0%) Frame = -3 Query 168 QVDEKGKNVTWQAMDYIGPDPDLQGKKNQEEEKCKGPLHSILVHINTKPHQAILDQLQQT 227 QVDEKGKNVTWQAMDYIGPDPDLQGKKNQEEEKCKGPLHSILVHINTKPHQAILDQLQQT Sbjct 9105722 QVDEKGKNVTWQAMDYIGPDPDLQGKKNQEEEKCKGPLHSILVHINTKPHQAILDQLQQT 9105543 Query 228 GFPAPTLSSSNLLTIHCDaviigsgsggsvaasvLAKSGHKVIVVEKGHYYQTTDLSLLE 287 GFPAPTLSSSNLLTIHCDAVIIGSGSGGSVAASVLAKSGHKVIVVEKGHYYQTTDLSLLE Sbjct 9105542 GFPAPTLSSSNLLTIHCDAVIIGSGSGGSVAASVLAKSGHKVIVVEKGHYYQTTDLSLLE 9105363 Query 288 GPTASVMYEGGGVIATDNIGVVMLAGSTIGGGSTINWSASIRTPDYVIKNWCHDHGLELF 347 GPTASVMYEGGGVIATDNIGVVMLAGSTIGGGSTINWSASIRTPDYVIKNWCHDHGLELF Sbjct 9105362 GPTASVMYEGGGVIATDNIGVVMLAGSTIGGGSTINWSASIRTPDYVIKNWCHDHGLELF 9105183 Query 348 SSSAYQRALDSVCERMSVQPHVLKEGFNNAVLRKGCAQLGIPVTNVPCNAPSDHYCGWCH 407 SSSAYQRALDSVCERMSVQPHVLKEGFNNAVLRKGCAQLGIPVTNVPCNAPSDHYCGWCH Sbjct 9105182 SSSAYQRALDSVCERMSVQPHVLKEGFNNAVLRKGCAQLGIPVTNVPCNAPSDHYCGWCH 9105003 Query 408 LGCKDAKKKSAQETWLVDLVESGNGLIIPGCSVLKVLHKIKNGCNRSEATGVVVQLEQYD 467 LGCKDAKKKSAQETWLVDLVESGNGLIIPGCSVLKVLHKIKNGCNRSEATGVVVQLEQYD Sbjct 9105002 LGCKDAKKKSAQETWLVDLVESGNGLIIPGCSVLKVLHKIKNGCNRSEATGVVVQLEQYD 9104823 Query 468 QQQAFVIESKITIVACGALNTPPLLKRSGLKNKHIGKNLHVHPCAMAWGYFPEEQQGWPE 527 QQQAFVIESKITIVACGALNTPPLLKRSGLKNKHIGKNLHVHPCAMAWGYFPEEQQGWPE Sbjct 9104822 QQQAFVIESKITIVACGALNTPPLLKRSGLKNKHIGKNLHVHPCAMAWGYFPEEQQGWPE 9104643 Query 528 KTMRSYEGAIITAMASVVSKQDGYGVILQTPALHPGMFSVLMPWISAQDFRERMRRFSRT 587 KTMRSYEGAIITAMASVVSKQDGYGVILQTPALHPGMFSVLMPWISAQDFRERMRRFSRT Sbjct 9104642 KTMRSYEGAIITAMASVVSKQDGYGVILQTPALHPGMFSVLMPWISAQDFRERMRRFSRT 9104463 Query 588 AHVFALARDKGAGMTDYPKSLTYKLADSDEENLREGLVMALKILAAAGAEEIGTHHCDGE 647 AHVFALARDKGAGMTDYPKSLTYKLADSDEENLREGLVMALKILAAAGAEEIGTHHCDGE Sbjct 9104462 AHVFALARDKGAGMTDYPKSLTYKLADSDEENLREGLVMALKILAAAGAEEIGTHHCDGE 9104283 Query 648 RFRVKGSEKNKFDEYLVRVRKMKRRNVWTPISTAHQMGSCRMGVDQKTSAVSPDGETWEV 707 RFRVKGSEKNKFDEYLVRVRKMKRRNVWTPISTAHQMGSCRMGVDQKTSAVSPDGETWEV Sbjct 9104282 RFRVKGSEKNKFDEYLVRVRKMKRRNVWTPISTAHQMGSCRMGVDQKTSAVSPDGETWEV 9104103 Query 708 EGLFVADTSVFPTALGVNPMVTVMAIAHCIANSALRFLERKI 749 EGLFVADTSVFPTALGVNPMVTVMAIAHCIANSALRFLERKI Sbjct 9104102 EGLFVADTSVFPTALGVNPMVTVMAIAHCIANSALRFLERKI 9103977 Score = 316 bits (810), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 169/198 (85%), Positives = 169/198 (85%), Gaps = 29/198 (15%) Frame = -1 Query 1 MAPPKVHDVSNDESGKRWSLWSGRPFGPNKLFKKEQESLAALCDALLPSMDLSNSNTKEE 60 MAPPKVHDVSNDESGKRWSLWSGRPFGPNKLFKKEQESLAALCDALLPSMDLSNSNTKEE Sbjct 9106426 MAPPKVHDVSNDESGKRWSLWSGRPFGPNKLFKKEQESLAALCDALLPSMDLSNSNTKEE 9106247 Query 61 AVSEFYTTSASMTGTPDM-----------------------------IGSMLAGEFQHPA 91 AVSEFYTTSASMTGTPDM IGSMLAGEFQHPA Sbjct 9106246 AVSEFYTTSASMTGTPDMVIYHFVELWNNIKFLSMM*FSVFLFVFYQIGSMLAGEFQHPA 9106067 Query 92 VPllrlallllSTWYGTFILCGRDSLSDKFPYFQKFSKVDVSKREQILLSWSLSPFALIR 151 VPLLRLALLLLSTWYGTFILCGRDSLSDKFPYFQKFSKVDVSKREQILLSWSLSPFALIR Sbjct 9106066 VPLLRLALLLLSTWYGTFILCGRDSLSDKFPYFQKFSKVDVSKREQILLSWSLSPFALIR 9105887 Query 152 MLFKAIKFITMRLFFSQV 169 MLFKAIKFITMRLFFSQV Sbjct 9105886 MLFKAIKFITMRLFFSQV 9105833 > Dr00002 on BDMI01000002.1 Dioscorea rotundata DNA, contig: Chromosome_02_P1_2_P2_2, cultivar: TDr96_F1, whole genome shotgun sequence Length=33969142 Score = 629 bits (1622), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/590 (54%), Positives = 429/590 (73%), Gaps = 14/590 (2%) Frame = -1 Query 168 QVDEKGKNVTWQAMDYIGPDPDL--QGKKNQEEEKCK----GPLHSILVHINTKPHQAIL 221 Q+DE +N TW+A+ Y GPDPD + K++ E E+ + GPLH LVH+++ P + + Sbjct 2016376 QLDENNENPTWKAIGYCGPDPDHVDRVKQSNESEQAQVDGLGPLHXTLVHLDS-PIETLS 2016200 Query 222 DQLQQTGFPAPTLSSSNLLTIHCDaviigsgsggsvaasvLAKSGHKVIVVEKGHYYQTT 281 L Q + T + L TI CD V++GSGSGG V A VLA++G+KV+V+EKGHY+ Sbjct 2016199 KALAQAXL-SITSRPNPLPTIRCDVVVVGSGSGGGVVAGVLAQAGYKVVVLEKGHYHARN 2016023 Query 282 DLSLLEGPTASVMYEGGGVIATDNIGVVMLAGSTIGGGSTINWSASIRTPDYVIKNWCHD 341 LSL+EGP+ MYE G++AT ++GV +LAGST+GGGS INWSASIRTPD V+ W Sbjct 2016022 KLSLIEGPSLDQMYERNGLLATADMGVTILAGSTVGGGSAINWSASIRTPDKVMGEWREK 2015843 Query 342 HGLELFSSSAYQRALDSVCERMSVQPHVLKEGFNNAVLRKGCAQLGIPVTNVPCNAPSDH 401 GLELF S AY+ ALD VC+RM V P V +E NNAVLR+GC +LG PV N+P NAPSDH Sbjct 2015842 LGLELFGSEAYEHALDVVCKRMGVHPDVEEESLNNAVLRRGCKELGYPVKNIPRNAPSDH 2015663 Query 402 YCGWCHLGCKDAKKKSAQETWLVDLVESGNGLIIPGCSVLKVLHKIKNGCNRSEATGVVV 461 +CGWC GCKD +KK ETWLVD+VESGNG+IIPG ++V+++ G + ATGVV Sbjct 2015662 HCGWCCFGCKDGRKKGTLETWLVDMVESGNGVIIPGSKAIRVIYE-NRGREKKVATGVVF 2015486 Query 462 QLEQ--YDQQQAFVIESKITIVACGALNTPPLLKRSGLKNKHIGKNLHVHPCAMAWGYFP 519 + E +++ ++E+K+T+VACGALNTP LLK+SGL+NK+IGK+LH+HP M WGYFP Sbjct 2015485 EFENGWSGEKEKCMVEAKVTVVACGALNTPVLLKKSGLRNKNIGKHLHLHPVVMGWGYFP 2015306 Query 520 EEQQGWPEKTMRSYEGAIITAMASVVSKQDGYGVILQTPALHPGMFSVLMPWISAQDFRE 579 E++ W + SYEGAI+TAM +VV +DGYG I+QTP+LHPG+FSV+ PW+S +DF+ Sbjct 2015305 EKEARW-DVDKNSYEGAIMTAMHTVV--RDGYGAIIQTPSLHPGLFSVVTPWLSGEDFKW 2015135 Query 580 RMRRFSRTAHVFALARDKGAGMTDYPKSLTYKLADSDEENLREGLVMALKILAAAGAEEI 639 RM RF+RTAH+F LARD+G G D S+ Y++ DE NL++G+ ++I+AAAGA EI Sbjct 2015134 RMTRFARTAHLFTLARDRGEGEVDESGSIRYRMDGHDERNLKKGVEKMMRIIAAAGATEI 2014955 Query 640 GTHHCDGERFRVKGSEKNKFDEYLVRVRKMKRRNVWTPISTAHQMGSCRMGVDQKTSAVS 699 GT H +GE+ ++ + ++F+ ++ R+ + + + TP+ +AHQMGSCRMGV +TSAV Sbjct 2014954 GTVHKEGEKLDLRTASSHEFERFVRRMGRKRVTGLRTPVCSAHQMGSCRMGVRPETSAVD 2014775 Query 700 PDGETWEVEGLFVADTSVFPTALGVNPMVTVMAIAHCIANSALRFLERKI 749 P GETWEV+GL+VADTSVFPTALGVNPMVTV AIA+C A S L RK+ Sbjct 2014774 PKGETWEVQGLYVADTSVFPTALGVNPMVTVQAIAYCTALSVAEVLRRKV 2014625 Score = 469 bits (1206), Expect = 1e-141, Method: Compositional matrix adjust. Identities = 250/605 (41%), Positives = 368/605 (61%), Gaps = 19/605 (3%) Frame = -2 Query 151 RMLFKAIKFIT--MRLFFSQVDEKGKNVTWQAMDYIGPDPDLQGKKNQEEEKCKGPLHSI 208 R++ + K +T +L Q +E +N +W+A+ Y P K ++ +E+ + PL Sbjct 31762329 RIIMHSRKXLT*FCKLLQMQTNEHSENPSWKAIGYY--LPTTSKKTSETQERRERPLEKG 31762156 Query 209 LVHINTKPHQAILDQLQQTGFPAPTLSSSNLLTIHCDaviigsgsggsvaasvLAKSGHK 268 ++ + + ++ + L + G S+ NL I CD VI+GSG GG VAA+VLA GHK Sbjct 31762155 IIESINQTNSSLFESLTEKGLKVTENSNENLYRIECDVVIVGSGCGGGVAAAVLASKGHK 31761976 Query 269 VIVVEKGHYYQTTDLSLLEGPTASVMYEGGGVIATDNIGVVMLAGSTIGGGSTINWSASI 328 V+V+EKG+Y+ + D + +EGP+ +YE GG ++ + ++++AG+ +GGGS +NWSA I Sbjct 31761975 VVVIEKGNYFTSDDYTSIEGPSLDQLYECGGKFSSLDGKMMLMAGTAVGGGSAVNWSACI 31761796 Query 329 RTPDYVIKNWCHDHGLELFSSSAYQRALDSVCERMSVQPHVLKEGFNNAVLRKGCAQLGI 388 RTP +V+K W +H L LFSSS Y A+D+VC+R+ V +EGF N +LRKGC LG+ Sbjct 31761795 RTPGHVLKEWSEEHHLRLFSSSXYXSAMDAVCQRIGVTERCTEEGFQNKILRKGCENLGL 31761616 Query 389 PVTNVPCNAPSDHYCGWCHLGCKDAKKKSAQETWLVDLVESGNGLIIPGCSVLKVL---H 445 V +VP N P +HYCG C GC+ +K TWLVD V +G +II C K+L + Sbjct 31761615 DVESVPRNCPENHYCGACCFGCRTGEKXGTDTTWLVDAVXNGX-VIITXCKAEKLLLEKN 31761439 Query 446 KIKNGCNRSEATGVVVQLEQYDQQQAFVIESKITIVACGALNTPPLLKRSGLKNKHIGKN 505 + + G+ + + SKITI ACG+L TP LL SGLKN +IGKN Sbjct 31761438 STXGDSKKMKCVGLXASFSSNAVTKKLEVRSKITISACGSLFTPTLLMASGLKNSNIGKN 31761259 Query 506 LHVHPCAMAWGYFPEEQQGWPEKTMRSYEGAIITAMASVVSKQDGYGVILQTPALHPGMF 565 LH+HP AWGYFP++ E R +EG IIT++ V + + I++ P++ P F Sbjct 31761258 LHLHPVIFAWGYFPDKVS---EIEGRVFEGGIITSLHKVANGR----AIIEXPSVGPASF 31761100 Query 566 SVLMPWISAQDFRERMRRFSRTAHVFALARDKGAGMTDYPKSLTYKLADSDEENLREGLV 625 + + PW S +D +E M ++SRTAH+FAL RD+G+G ++Y DEENL GL Sbjct 31761099 ASIFPWNSGRDMKEMMLKYSRTAHLFALVRDQGSGSVRGEGRISYAFDKFDEENLVVGLR 31760920 Query 626 MALKILAAAGAEEIGTHHCDGERFRVKGSEKNKFDEYLVRV----RKMKRRNVWTPISTA 681 AL+IL AAGA E+GT+ DG+R + KG ++ + +E+L V +W+ TA Sbjct 31760919 TALRILVAAGAVEVGTYRSDGQRMKCKGIKEEELEEFLDGVVCAGGPKSGEELWSMYPTA 31760740 Query 682 HQMGSCRMGVDQKTSAVSPDGETWEVEGLFVADTSVFPTALGVNPMVTVMAIAHCIANSA 741 HQMGSCRMGV ++ V +GE+WE +GLFV D S+FPTALGVNPM+T+ +IA+C++N Sbjct 31760739 HQMGSCRMGVSEEKGGVDENGESWEAKGLFVCDGSLFPTALGVNPMITIQSIAYCLSNKI 31760560 Query 742 LRFLE 746 L+ Sbjct 31760559 AESLQ 31760545 Score = 53.1 bits (126), Expect = 9e-06, Method: Compositional matrix adjust. Identities = 30/82 (37%), Positives = 44/82 (54%), Gaps = 1/82 (1%) Frame = -1 Query 103 STWYGTFILCGRDSLSDKFPYFQKFSKVDVSKREQILLSWSLSP-FALIRMLFKAIKFIT 161 ST GT +LCG SL FP+ KFS + + KRE++L W+ F +RM+F +K I Sbjct 31763251 STRLGTLVLCGTLSLHRGFPFINKFSDMSLDKREEVLKRWTKGKCFKTLRMVFLMVKIIC 31763072 Query 162 MRLFFSQVDEKGKNVTWQAMDY 183 +FFS V + + Q + Sbjct 31763071 FFVFFSMVIDSSPQNSXQNFSF 31763006 Score = 36.2 bits (82), Expect = 1.2, Method: Compositional matrix adjust. Identities = 15/60 (25%), Positives = 32/60 (53%), Gaps = 8/60 (13%) Frame = -3 Query 679 STAHQMGSCRMGVDQKTSAVSPDGETWEV--------EGLFVADTSVFPTALGVNPMVTV 730 + H +G + ++ +P + ++V +GL+V D+S+ P +G+NP+VT+ Sbjct 29854778 TLVHLLGG*NVCLNPSNGVCNPKSQVFDVSGDQPLVHKGLYVCDSSLIPCLVGINPIVTI 29854599 Score = 34.3 bits (77), Expect = 5.2, Method: Compositional matrix adjust. Identities = 20/49 (41%), Positives = 31/49 (63%), Gaps = 7/49 (14%) Frame = -2 Query 37 ESLAALCDALLPSM-DLSN--SNTKEEA----VSEFYTTSASMTGTPDM 78 +SL+A+C+A +PS+ D++ S+ KEE+ + FY SAS PDM Sbjct 31763787 QSLSAMCEAFIPSLPDMAAHVSSGKEESLCKNIQAFYLASASQAPIPDM 31763641 > Dr00002 on BDMI01000004.1 Dioscorea rotundata DNA, contig: Chromosome_04_P1_4_P2_5, cultivar: TDr96_F1, whole genome shotgun sequence Length=27749968 Score = 88.2 bits (217), Expect(2) = 4e-24, Method: Compositional matrix adjust. Identities = 49/93 (53%), Positives = 66/93 (71%), Gaps = 0/93 (0%) Frame = +3 Query 77 DMIGSMLAGEFQHPAVPllrlallllSTWYGTFILCGRDSLSDKFPYFQKFSKVDVSKRE 136 + +G L+G+ QH + LLRL L LLSTWYGT ILCG S+S +FPYF KFS++++ +RE Sbjct 3368574 EQVGVYLSGKLQHVMLGLLRLGLWLLSTWYGTLILCGYKSMSSRFPYFHKFSQLELRRRE 3368753 Query 137 QILLSWSLSPFALIRMLFKAIKFITMRLFFSQV 169 +I+ SWS S L+R LF +KFI M F+QV Sbjct 3368754 EIMKSWSSSYIYLLRQLFVGVKFIVMLAHFTQV 3368852 Score = 46.6 bits (109), Expect(2) = 4e-24, Method: Compositional matrix adjust. Identities = 27/72 (38%), Positives = 41/72 (57%), Gaps = 1/72 (1%) Frame = +1 Query 13 ESGKRWSLWSGRPFGPNKLFKKEQESLAALCDALLPSMDLSNSNTKEEAVSEFYTTSASM 72 E+ R+S + NKL +E ESL A+ D LPS+ NS ++ F++TSASM Sbjct 3368287 EAALRFSNGVSQAPPVNKLTAREMESLIAISDTFLPSIQ-XNSANYSTSLQTFFSTSASM 3368463 Query 73 TGTPDMIGSMLA 84 GTP+++ L+ Sbjct 3368464 LGTPEVVSIYLS 3368499 Score = 45.4 bits (106), Expect = 0.002, Method: Compositional matrix adjust. Identities = 33/137 (24%), Positives = 60/137 (44%), Gaps = 24/137 (18%) Frame = -3 Query 625 VMALKILAAAGAEEIGTHHCDGERFRVKGSEKNKF----DEYLVRVRKMKRRNVWTPI-- 678 +M LK A +G CDG K + K +F D L+R + +++ + Sbjct 18272048 LMGLKASQAMVLNVMGYDSCDGRITFDKETNKIRFTQAHDPLLLR-KIQALQSIXKKLGG 18271872 Query 679 ---------STAHQMGSCRMGVDQKTSAVSPDGETWEV--------EGLFVADTSVFPTA 721 ++ H +G C D +P G+ ++V EGL+V D S+ P + Sbjct 18271871 MLFMSRYRSTSVHLLGGCNAASDSLNGVCNPKGQVFDVSGDQPARHEGLYVCDGSLIPCS 18271692 Query 722 LGVNPMVTVMAIAHCIA 738 +G+NP++T+ + I+ Sbjct 18271691 VGINPILTITTTSEYIS 18271641 Score = 44.7 bits (104), Expect = 0.003, Method: Compositional matrix adjust. Identities = 33/145 (23%), Positives = 61/145 (42%), Gaps = 30/145 (21%) Frame = -1 Query 625 VMALKILAAAGAEEIGTHHCDGERFRVKGSEKNKF----DEYLVR--------------V 666 +M LK A +G CDG K + K +F D L+R + Sbjct 18244786 LMGLKASQAMVLNVMGYDSCDGRITFDKETNKIRFTQAHDPLLLRKIQALQSISKKLGGI 18244607 Query 667 RKMKRRNVWTPISTAHQMGSCRMGVDQKTSAVSPDGETWEV--------EGLFVADTSVF 718 M R ++ H +G C +D +P G+ +++ +GL+V D S+ Sbjct 18244606 LFMSRYRS----TSVHLLGGCNAALDPSNGVCNPKGQVFDMSGDQPAVHKGLYVCDASLI 18244439 Query 719 PTALGVNPMVTVMAIAHCIANSALR 743 P ++G+NP++T+ + I+ ++ Sbjct 18244438 PCSIGINPILTITTASEYISKHLVK 18244364 Lambda K H a alpha 0.319 0.133 0.409 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 94682285528 Database: Dioscorea_rotundata.fa Posted date: Jan 8, 2019 5:53 PM Number of letters in database: 456,674,974 Number of sequences in database: 21 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40