BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Dendrobium_officinale.pep 35,567 sequences; 11,846,126 total letters Query= Dendrobium_GLEAN_10145763 [mRNA] [translate_table: standard] Length=214 Score E Sequences producing significant alignments: (Bits) Value Dendrobium_GLEAN_10145763 [mRNA] [translate_table: standard] 422 4e-151 Dendrobium_GLEAN_10026641 [mRNA] [translate_table: standard] 154 3e-46 Dendrobium_GLEAN_10113180 [mRNA] [translate_table: standard] 154 5e-46 Dendrobium_GLEAN_10054343 [mRNA] [translate_table: standard] 89.7 1e-22 Dendrobium_GLEAN_10118536 [mRNA] [translate_table: standard] 32.7 0.11 Dendrobium_GLEAN_10030212 [mRNA] [translate_table: standard] 32.0 0.17 Dendrobium_GLEAN_10062264 [mRNA] [translate_table: standard] 28.1 3.6 Dendrobium_GLEAN_10133544 [mRNA] [translate_table: standard] 28.1 3.7 Dendrobium_GLEAN_10005670 [mRNA] [translate_table: standard] 27.7 3.9 Dendrobium_GLEAN_10120411 [mRNA] [translate_table: standard] 27.7 6.5 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10145763 [mRNA] [translate_table: standard] Length=214 Score = 422 bits (1085), Expect = 4e-151, Method: Compositional matrix adjust. Identities = 214/214 (100%), Positives = 214/214 (100%), Gaps = 0/214 (0%) Query 1 MAIPITSLSSSASSRHHTHQAYLLTNYLLIGAASSCIFLTLSLRLLPSPCGLLLISLHSL 60 MAIPITSLSSSASSRHHTHQAYLLTNYLLIGAASSCIFLTLSLRLLPSPCGLLLISLHSL Sbjct 1 MAIPITSLSSSASSRHHTHQAYLLTNYLLIGAASSCIFLTLSLRLLPSPCGLLLISLHSL 60 Query 61 TAIAAASAAASPVASSDRSHAAHTAAAALTAIFHGATALLAFTRSPDFIAEIRSYVREDD 120 TAIAAASAAASPVASSDRSHAAHTAAAALTAIFHGATALLAFTRSPDFIAEIRSYVREDD Sbjct 61 TAIAAASAAASPVASSDRSHAAHTAAAALTAIFHGATALLAFTRSPDFIAEIRSYVREDD 120 Query 121 AIVILKLVGGLCGAIFCLEWVAMALAFALRFDDGEDRDCSTEKRVGRVEKREERLGLGRI 180 AIVILKLVGGLCGAIFCLEWVAMALAFALRFDDGEDRDCSTEKRVGRVEKREERLGLGRI Sbjct 121 AIVILKLVGGLCGAIFCLEWVAMALAFALRFDDGEDRDCSTEKRVGRVEKREERLGLGRI 180 Query 181 FESSSSFLLGWMRSKEAAAWRRERRVALVPFSRP 214 FESSSSFLLGWMRSKEAAAWRRERRVALVPFSRP Sbjct 181 FESSSSFLLGWMRSKEAAAWRRERRVALVPFSRP 214 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10026641 [mRNA] [translate_table: standard] Length=190 Score = 154 bits (389), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 80/164 (49%), Positives = 111/164 (68%), Gaps = 7/164 (4%) Query 12 ASSRHHTHQAYLLTNYLLIGAASSCIFLTLSLRLLPSPCGLLLISLHSLTAIAAASAAAS 71 +++ + TH+ +LL+NY+ +GAASSCIFLTLSLRL+PS G LLI L +LT A S A+ Sbjct 2 SAAHYRTHKIFLLSNYIFLGAASSCIFLTLSLRLIPSTIGFLLILLQALTIAGAISGCAA 61 Query 72 PVASSDRSHAAHTAAAALTAIFHGATALLAFTRSPDF-IAEIRSYVREDDAIVILKLVGG 130 ++S++ + H A LTAI GA A+L F+R+ +F ++ +RSYVR DD +IL++VGG Sbjct 62 ANSTSNKWYDTHMVATVLTAILQGAVAILVFSRTSEFLLSGLRSYVRVDDGEIILRMVGG 121 Query 131 LCGAIFCLEWVAMALAFALRFDDGEDRDCSTEKRVGRVEKREER 174 L AIFCLEWV +ALAF LR+ D DC GR +E+ Sbjct 122 LRVAIFCLEWVVLALAFVLRYYAYVDGDC------GRRNAKEDN 159 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10113180 [mRNA] [translate_table: standard] Length=193 Score = 154 bits (388), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 85/175 (49%), Positives = 117/175 (67%), Gaps = 14/175 (8%) Query 1 MAIPITSLSSSASSRHHTHQAYLLTNYLLIGAASSCIFLTLSLRLLPSPCGLLLISLHSL 60 M + T+ S +++ + TH+ +LL+NY+L+GAASSCIFLTLSLRL+PS G LLI L +L Sbjct 1 MGLSSTASSDMSAAHYRTHKIFLLSNYILLGAASSCIFLTLSLRLIPSAIGFLLILLQAL 60 Query 61 TAIAAASAAASPVASSDRSHAAHTAAAALTAIFHGATALLAFTRSPDF-IAEIRSYVRED 119 T IAAA+ ++S++ + H A LTAI GA A+L F+R+ +F + +RSYVR D Sbjct 61 T-IAAAN------STSNKWYGTHMVATVLTAILQGAVAILVFSRTSEFLLGGLRSYVRVD 113 Query 120 DAIVILKLVGGLCGAIFCLEWVAMALAFALRFDDGEDRDCSTEKRVGRVEKREER 174 D +IL++VGGL AIFCLEWV +ALAF LR+ D DC GR +E+ Sbjct 114 DGEIILRMVGGLRVAIFCLEWVVLALAFVLRYYAYVDGDC------GRRNAKEDN 162 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10054343 [mRNA] [translate_table: standard] Length=103 Score = 89.7 bits (221), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 51/101 (50%), Positives = 68/101 (67%), Gaps = 5/101 (5%) Query 8 LSSSASS-----RHHTHQAYLLTNYLLIGAASSCIFLTLSLRLLPSPCGLLLISLHSLTA 62 LSS+ASS + TH+ +LL+NY+ +GAASSCIFLTLSLRL+PS G LLI L +LT Sbjct 3 LSSTASSDMSAAHYRTHKIFLLSNYIFLGAASSCIFLTLSLRLIPSTIGFLLILLQALTI 62 Query 63 IAAASAAASPVASSDRSHAAHTAAAALTAIFHGATALLAFT 103 A S A+ ++S++ + H A LTAI GA A+L F+ Sbjct 63 AGAISGCAAANSTSNKWYDTHMVATVLTAILQGAVAILVFS 103 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10118536 [mRNA] [translate_table: standard] Length=355 Score = 32.7 bits (73), Expect = 0.11, Method: Compositional matrix adjust. Identities = 13/34 (38%), Positives = 20/34 (59%), Gaps = 1/34 (3%) Query 168 VEKREERLGLGRIFESSSSF-LLGWMRSKEAAAW 200 K++ +LGL +IF + SF L+GW + K W Sbjct 226 TNKKQPQLGLAKIFAQAKSFGLVGWAKPKPKLGW 259 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10030212 [mRNA] [translate_table: standard] Length=192 Score = 32.0 bits (71), Expect = 0.17, Method: Compositional matrix adjust. Identities = 21/91 (23%), Positives = 41/91 (45%), Gaps = 8/91 (9%) Query 39 LTLSLRLLPSPCG----LLLISLHSLTAIAAASAAASPVASSDRSHAAHTAAA-ALTAIF 93 L S+ PSPCG L+ I +HS + ++ V +++ H A + +F Sbjct 58 LHFSMDPNPSPCGSGEALVTIDVHSTKTLMSSGHRYLDVRTAEEFQKGHPEKALNVPYMF 117 Query 94 HGATALLAFTRSPDFIAEIRSYVREDDAIVI 124 ++ +P+F+ E+ +DD I++ Sbjct 118 FAPQGRVS---NPNFVEEVTDLCSKDDFIIV 145 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10062264 [mRNA] [translate_table: standard] Length=244 Score = 28.1 bits (61), Expect = 3.6, Method: Compositional matrix adjust. Identities = 20/58 (34%), Positives = 30/58 (52%), Gaps = 7/58 (12%) Query 163 KRVGRVEKREER-LGLGRIFES---SSSFLLGWMRSKEAAAWRRERRVAL---VPFSR 213 K++ E +E R GL R FE S+ +LGW+ S +A W+ + AL VP + Sbjct 82 KKLTETELQENRAKGLRREFEPVSIGSTSVLGWLFSSDAGFWKPPQMKALLHSVPIGK 139 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10133544 [mRNA] [translate_table: standard] Length=421 Score = 28.1 bits (61), Expect = 3.7, Method: Compositional matrix adjust. Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 0/42 (0%) Query 156 DRDCSTEKRVGRVEKREERLGLGRIFESSSSFLLGWMRSKEA 197 DRD S + E++ LGL RI S F L W +KEA Sbjct 123 DRDRSMDGSSDLDERKWGHLGLTRITPMGSDFFLCWFENKEA 164 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10005670 [mRNA] [translate_table: standard] Length=196 Score = 27.7 bits (60), Expect = 3.9, Method: Compositional matrix adjust. Identities = 32/122 (26%), Positives = 44/122 (36%), Gaps = 31/122 (25%) Query 68 AAASPVASSDRSHAAHTAAAA-----LTAIFHGATALLAF----------------TRSP 106 +A P S A AAA +TAI GAT+ + F R+P Sbjct 11 GSAGPSGFGSTSTAVDVTAAANHLGAITAIITGATSGIGFETARALAARGARLILPARNP 70 Query 107 DFIAEIRSYVREDDAIVILKLVGGLCGAIFCLEWVAMALAFALR----------FDDGED 156 D E++S + I+IL L G +I +AL F L +D E Sbjct 71 DAAEEVKSRIAGGGQIIILPLDLGSLSSIRSFVSQFLALGFPLNILINNAGRFAYDQAES 130 Query 157 RD 158 D Sbjct 131 ED 132 > Dendrobium_GLEAN_10145763 on Dendrobium_GLEAN_10120411 [mRNA] [translate_table: standard] Length=709 Score = 27.7 bits (60), Expect = 6.5, Method: Composition-based stats. Identities = 26/100 (26%), Positives = 46/100 (46%), Gaps = 15/100 (15%) Query 31 GAASSCIFLTLSLRLLPSPCGLLLISLHSLTAIAAASAAASPVASSDRSHAAHTAAAALT 90 G S+ +FL++ PC +LLI++ +L A AA +P+ H A T + T Sbjct 186 GTLSAFLFLSV-------PCWVLLITMEALPKNPANFAALTPLTF---LHRAATVYSTRT 235 Query 91 AIFHGATALL---AFTRSPDFIAEIRS--YVREDDAIVIL 125 +I + T R A +RS ++ ++D + +L Sbjct 236 SIIYNRTHFTWEQTHIRCRRLAAALRSLLHISKNDVVSVL 275 Lambda K H a alpha 0.324 0.133 0.391 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 1020339360 Database: Dendrobium_officinale.pep Posted date: Jan 8, 2019 11:03 AM Number of letters in database: 11,846,126 Number of sequences in database: 35,567 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40