BLAST Results
BLASTX 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Calotropis_gigantea.pep 20,832 sequences; 9,345,609 total letters Query= cal_g013864.t1 Length=960 Score E Sequences producing significant alignments: (Bits) Value cal_g013864.t1 655 0.0 cal_g015794.t1 40.0 0.001 cal_g014825.t1 30.0 0.70 cal_g004166.t1 30.0 1.6 cal_g012152.t1 30.0 1.7 cal_g006241.t1 30.0 1.9 cal_g012602.t1 28.1 7.5 > cal_g013864.t1 on cal_g013864.t1 Length=685 Score = 655 bits (1689), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/320 (100%), Positives = 320/320 (100%), Gaps = 0/320 (0%) Frame = +1 Query 1 MEVSFVSKARTAFHSAAAKAEKVFADIKKSDSSADRAEHSNAQLQKELTPDSVNTKEDDS 180 MEVSFVSKARTAFHSAAAKAEKVFADIKKSDSSADRAEHSNAQLQKELTPDSVNTKEDDS Sbjct 1 MEVSFVSKARTAFHSAAAKAEKVFADIKKSDSSADRAEHSNAQLQKELTPDSVNTKEDDS 60 Query 181 EYCGGTKQSKRKPPPIKTKQDWQERFKNIRIGKRGNEDTEKSENSKMAYAIFDENLYLIR 360 EYCGGTKQSKRKPPPIKTKQDWQERFKNIRIGKRGNEDTEKSENSKMAYAIFDENLYLIR Sbjct 61 EYCGGTKQSKRKPPPIKTKQDWQERFKNIRIGKRGNEDTEKSENSKMAYAIFDENLYLIR 120 Query 361 EREVFHSEESKSGSGGQDFDSSNANIIPSTSVIRQLAVAIETSKNYNSMMDLMASSRDSS 540 EREVFHSEESKSGSGGQDFDSSNANIIPSTSVIRQLAVAIETSKNYNSMMDLMASSRDSS Sbjct 121 EREVFHSEESKSGSGGQDFDSSNANIIPSTSVIRQLAVAIETSKNYNSMMDLMASSRDSS 180 Query 541 PVRERAALGFSAVKSLVLREKEEKLVTEFGTNEKVLSLIKSLLHAEGQSLIGRDEREAGE 720 PVRERAALGFSAVKSLVLREKEEKLVTEFGTNEKVLSLIKSLLHAEGQSLIGRDEREAGE Sbjct 181 PVRERAALGFSAVKSLVLREKEEKLVTEFGTNEKVLSLIKSLLHAEGQSLIGRDEREAGE 240 Query 721 ITNMSSLIKDIHGAPPESFVVKLSEAIGSLKTLRKMASFWSRVVTEMRRLWSEWKYIPGI 900 ITNMSSLIKDIHGAPPESFVVKLSEAIGSLKTLRKMASFWSRVVTEMRRLWSEWKYIPGI Sbjct 241 ITNMSSLIKDIHGAPPESFVVKLSEAIGSLKTLRKMASFWSRVVTEMRRLWSEWKYIPGI 300 Query 901 PPDEVPDLNSCLLYQQLQVI 960 PPDEVPDLNSCLLYQQLQVI Sbjct 301 PPDEVPDLNSCLLYQQLQVI 320 > cal_g013864.t1 on cal_g015794.t1 Length=939 Score = 40.0 bits (92), Expect = 0.001, Method: Compositional matrix adjust. Identities = 42/178 (24%), Positives = 80/178 (45%), Gaps = 20/178 (11%) Frame = +1 Query 436 IIPSTSVIRQLAVAIETSKNYNSMMDLMASSRDSSPVRERAALGFSAVKSLVLREKEEKL 615 IIP S+ + + + + +++ + SS D + + ++ SA +L K + Sbjct 317 IIPDLSLTGSIGNFVGFASQLHLLVNALESSLDGQFMEDFVSVENSASDNL----KSSAV 372 Query 616 VTEFGTNEKVLSLIKSLLHAEGQSLIGRDEREAGEITNMSSLIKDIHGAPPESFVVKLSE 795 + + ++VL K L H E +L+ D E + I GAP +S + Sbjct 373 IPQPTVLDRVL---KDLFHEE--TLLELDLIEPKNS-------RAIKGAPLQSLFAQFCL 420 Query 796 A---IGSLKTLRKMASFWSRVVTEMRRLWSEWKYIPGIPPDEVPDLNSCLLYQQLQVI 960 G+ +R +A W V E+R W E + +P +P + DL++CL+ Q+L ++ Sbjct 421 HGLWFGNCN-IRAIAVLWIEFVREVRWCWEEKQPLPRMPVNGSIDLSTCLINQKLHML 477 > cal_g013864.t1 on cal_g014825.t1 Length=130 Score = 30.0 bits (66), Expect = 0.70, Method: Compositional matrix adjust. Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 0/37 (0%) Frame = -2 Query 734 DIFVISPASRSSLPIRDCPSACSSDLIKESTFSLVPN 624 IF+ P +RS DC + +KE+ FS++ N Sbjct 66 HIFLSCPYARSGWQFADCQTLMQKLQVKEANFSMIEN 102 > cal_g013864.t1 on cal_g004166.t1 Length=474 Score = 30.0 bits (66), Expect = 1.6, Method: Compositional matrix adjust. Identities = 14/39 (36%), Positives = 24/39 (62%), Gaps = 0/39 (0%) Frame = -2 Query 740 KDDIFVISPASRSSLPIRDCPSACSSDLIKESTFSLVPN 624 K D+ VI+P+S S +P+ DC S+ + E+ S++ N Sbjct 391 KADMIVINPSSWSMVPLHDCISSLVYSMRTENIVSVMCN 429 > cal_g013864.t1 on cal_g012152.t1 Length=448 Score = 30.0 bits (66), Expect = 1.7, Method: Compositional matrix adjust. Identities = 18/52 (35%), Positives = 29/52 (56%), Gaps = 1/52 (2%) Frame = +1 Query 502 SMMDLMASSRDSSPVRERAALGFSAVKSLVLREKEEKLVTEFGT-NEKVLSL 654 S+M + + +PVRE A L F +LVL + + K+ + GT N+ V+ L Sbjct 86 SIMRWVWDANRGNPVRENATLTFGRNGNLVLADVDGKVAWQTGTANKGVVGL 137 > cal_g013864.t1 on cal_g006241.t1 Length=472 Score = 30.0 bits (66), Expect = 1.9, Method: Compositional matrix adjust. Identities = 30/112 (27%), Positives = 52/112 (46%), Gaps = 11/112 (10%) Frame = +1 Query 220 PPIKTKQDWQERFKNIRIGKRGNEDTEKSENSKM-AY----AIFDENLYLIRE---REVF 375 P +++KQ E+ +N +IG G++D S M AY + D NL+LIR+ +F Sbjct 216 PCLRSKQ---EKLRNGKIGNSGDDDRLDCLTSLMEAYQRDAGVGDSNLFLIRDVLLNLMF 272 Query 376 HSEESKSGSGGQDFDSSNANIIPSTSVIRQLAVAIETSKNYNSMMDLMASSR 531 ++ S + F N + ++ I+ SKN + D+ SS+ Sbjct 273 AGRDTISITLSWLFWLLTKNPSVEAKIREEIENEIKDSKNSRYIFDMKVSSK 324 > cal_g013864.t1 on cal_g012602.t1 Length=583 Score = 28.1 bits (61), Expect = 7.5, Method: Compositional matrix adjust. Identities = 20/48 (42%), Positives = 26/48 (54%), Gaps = 2/48 (4%) Frame = +1 Query 304 SENSKMAYAIFDENLYLIRE-REVFHSEESKSG-SGGQDFDSSNANII 441 SEN K+ FD+ LI ++ H EE S S G FDS N+NI+ Sbjct 313 SENFKLMSPTFDKRDALITSMNQIHHKEEDASTISMGITFDSGNSNIL 360 Lambda K H a alpha 0.311 0.127 0.348 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 1621504206 Database: Calotropis_gigantea.pep Posted date: Jan 8, 2019 1:06 AM Number of letters in database: 9,345,609 Number of sequences in database: 20,832 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 12 Window for multiple hits: 40