BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Mentha_longifoli.pep 4,329 sequences; 1,311,348 total letters Query= WP_000003071.1 MULTISPECIES: lysine--tRNA ligase [Proteobacteria] Length=505 Score E Sequences producing significant alignments: (Bits) Value WP_000003071.1 MULTISPECIES: lysine--tRNA ligase [Proteobacteria] 1038 0.0 WP_001295090.1 MULTISPECIES: lysine--tRNA ligase heat inducible... 938 0.0 WP_000004771.1 MULTISPECIES: elongation factor P--(R)-beta-lysi... 140 5e-38 WP_001258678.1 MULTISPECIES: aspartate--tRNA ligase [Enterobact... 94.7 2e-21 WP_000117881.1 MULTISPECIES: asparagine--tRNA ligase [Proteobac... 63.9 1e-11 WP_000138915.1 MULTISPECIES: malate synthase A [Enterobacteriac... 30.8 0.20 WP_001301303.1 MULTISPECIES: IMPACT family member YigZ [Enterob... 28.1 1.0 WP_000187022.1 MULTISPECIES: tRNA (uridine(54)-C5)-methyltransf... 28.1 1.6 WP_000263098.1 MULTISPECIES: DNA-directed RNA polymerase subuni... 27.7 2.6 > WP_000003071.1 on WP_000003071.1 MULTISPECIES: lysine--tRNA ligase [Proteobacteria] Length=505 Score = 1038 bits (2685), Expect = 0.0, Method: Compositional matrix adjust. Identities = 505/505 (100%), Positives = 505/505 (100%), Gaps = 0/505 (0%) Query 1 MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEE 60 MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEE Sbjct 1 MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEE 60 Query 61 LEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGD 120 LEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGD Sbjct 61 LEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGD 120 Query 121 ILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDES 180 ILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDES Sbjct 121 ILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDES 180 Query 181 RNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP 240 RNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP Sbjct 181 RNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP 240 Query 241 ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRT 300 ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRT Sbjct 241 ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRT 300 Query 301 LAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGI 360 LAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGI Sbjct 301 LAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGI 360 Query 361 HVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG 420 HVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG Sbjct 361 HVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG 420 Query 421 REIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID 480 REIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID Sbjct 421 REIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID 480 Query 481 RMVMLFTNSHTIRDVILFPAMRPVK 505 RMVMLFTNSHTIRDVILFPAMRPVK Sbjct 481 RMVMLFTNSHTIRDVILFPAMRPVK 505 > WP_000003071.1 on WP_001295090.1 MULTISPECIES: lysine--tRNA ligase heat inducible [Enterobacteriaceae] Length=505 Score = 938 bits (2424), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/505 (89%), Positives = 479/505 (95%), Gaps = 0/505 (0%) Query 1 MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEE 60 MSEQ +GA+ +D N+EL+ RREKLA LR+QG+AFPNDFRRDHTS+QLH EFD K+N+E Sbjct 1 MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDHTSEQLHEEFDAKDNQE 60 Query 61 LEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGD 120 LE+LNIEV+VAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD LPEGVYN+QFKKWDLGD Sbjct 61 LESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDSLPEGVYNDQFKKWDLGD 120 Query 121 ILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDES 180 I+GA+G LFKT+TGELSIHCTELRLLTKALRPLPDKFHGLQDQE RYRQRYLDLI+ND+S Sbjct 121 IIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGLQDQEVRYRQRYLDLIANDKS 180 Query 181 RNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP 240 R TF VRS+IL+ IRQFMV RGFMEVETPMMQVIPGGA+ARPFITHHNALDLDMYLRIAP Sbjct 181 RQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGASARPFITHHNALDLDMYLRIAP 240 Query 241 ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRT 300 ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADY DLIELTESLFRT Sbjct 241 ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYHDLIELTESLFRT 300 Query 301 LAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGI 360 LAQ++LG T+VTYG+ DFGKPFEKLTMREAIKKYRPETDMADLDNFD+AKA+AESIGI Sbjct 301 LAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIKKYRPETDMADLDNFDAAKALAESIGI 360 Query 361 HVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG 420 VEKSWGLGRIVTEIF+EVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG Sbjct 361 TVEKSWGLGRIVTEIFDEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG 420 Query 421 REIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID 480 REIGNGFSELNDAEDQA+RF +QV AK AGDDEAMFYDEDYVTALE+GLPPTAGLGIGID Sbjct 421 REIGNGFSELNDAEDQAERFQEQVNAKAAGDDEAMFYDEDYVTALEYGLPPTAGLGIGID 480 Query 481 RMVMLFTNSHTIRDVILFPAMRPVK 505 RM+MLFTNSHTIRDVILFPAMRP K Sbjct 481 RMIMLFTNSHTIRDVILFPAMRPQK 505 > WP_000003071.1 on WP_000004771.1 MULTISPECIES: elongation factor P--(R)-beta-lysine ligase [Proteobacteria] Length=325 Score = 140 bits (353), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 98/321 (31%), Positives = 166/321 (52%), Gaps = 28/321 (9%) Query 187 RSQILSGIRQFMVNRGFMEVETPMM-QVIPGGAAARPFITHH----NALDLDMYLRIAPE 241 R+ I++ IR+F +RG +EVETP M Q PF T ++ ++++L +PE Sbjct 19 RAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPE 78 Query 242 LYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTL 301 ++KRL+V G VF++ R+FRNE + HNPEFTM+E Y + D L+ + L + + Sbjct 79 YHMKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQV 138 Query 302 AQDILGKTEVTYGDVTLDFGK--PF--EKLTMREAIKKYRPETDMADLDNFDSAKAIAES 357 D ++Y L + + P +K +RE K DL N + ++ Sbjct 139 L-DCPAAESLSYQQAFLRYLEIDPLSADKTQLREVAAKL-------DLSNVADTEEDRDT 190 Query 358 IGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFF 417 + + + ++G + I +E +PTF+ +PA + LA+ + + + +RFE + Sbjct 191 L-LQLLFTFG---VEPNIGKE-------KPTFVYHFPASQASLAQISTEDHRVAERFEVY 239 Query 418 IGGREIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGI 477 G E+ NGF EL DA +Q QRF + A D++ + AL+ G+P +G+ + Sbjct 240 YKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVAL 299 Query 478 GIDRMVMLFTNSHTIRDVILF 498 G+DR+VML + T+ +VI F Sbjct 300 GVDRLVMLALGAETLAEVIAF 320 > WP_000003071.1 on WP_001258678.1 MULTISPECIES: aspartate--tRNA ligase [Enterobacteriaceae] Length=590 Score = 94.7 bits (234), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 86/304 (28%), Positives = 149/304 (49%), Gaps = 35/304 (12%) Query 61 LEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD-----DLPEGVYNEQFKK 115 L + +V + G + RR +G F+ ++D G +Q++ D L + NE F Sbjct 11 LSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGIVQVFFDPDRADALKLASELRNE-FCI 69 Query 116 WDLGDILGAKGKLFK--TKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLD 173 G + K TGE+ + + L ++ +A LP + + +EAR + RYLD Sbjct 70 QVTGTVRARDEKNINRDMATGEIEVLASSLTIINRA-DVLPLDSNHVNTEEARLKYRYLD 128 Query 174 LISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMM-QVIPGGAAARPFITHHNALDL 232 L E K R++I S +R+FM + GF+++ETPM+ + P GA R ++ Sbjct 129 L-RRPEMAQRLKTRAKITSLVRRFMDDHGFLDIETPMLTKATPEGA--RDYLVPSRVHKG 185 Query 233 DMY-LRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLI 291 Y L +P+L+ + L++ GF+R ++I + FR+E + PEFT +++ ++ + Sbjct 186 KFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVR 245 Query 292 ELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKY---RPE-------TD 341 E+ E+L R L ++ G V GD F +T EA ++Y +P+ TD Sbjct 246 EVMEALVRHLWLEVKG---VDLGD--------FPVMTFAEAERRYGSDKPDLRNPMELTD 294 Query 342 MADL 345 +ADL Sbjct 295 VADL 298 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 39/147 (27%), Positives = 66/147 (45%), Gaps = 5/147 (3%) Query 354 IAESIGIHVEKSWG-LGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITD 412 + + +G+ E W L I +FE+ E L P +++ + + + Sbjct 413 VGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHPFTSPKDMTAAELKAAPENAVAN 472 Query 413 RFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPT 472 ++ I G E+G G +++ D Q + + E + + AL++G PP Sbjct 473 AYDMVINGYEVGGGSVRIHNG-DMQQTVFGILGINEEEQREKFGF---LLDALKYGTPPH 528 Query 473 AGLGIGIDRMVMLFTNSHTIRDVILFP 499 AGL G+DR+ ML T + IRDVI FP Sbjct 529 AGLAFGLDRLTMLLTGTDNIRDVIAFP 555 > WP_000003071.1 on WP_000117881.1 MULTISPECIES: asparagine--tRNA ligase [Proteobacteria] Length=466 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 101/472 (21%), Positives = 183/472 (39%), Gaps = 63/472 (13%) Query 63 ALNIEVAVAGRMMTRR-IMGKASFVTLQDVGG--RIQLYVARDDLPEGVYNEQFKKWDLG 119 A++ EV V G + TRR SF+ + D +Q V + LP YNE + G Sbjct 15 AVDSEVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQA-VINNSLPN--YNEDVLRLTTG 71 Query 120 DILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDE 179 + GK+ + ++ PD + R+ YL +++ Sbjct 72 CSVIVTGKVVASPGQGQQFEIQASKVEVAGWVEDPDTY---PMAAKRHSIEYLREVAHLR 128 Query 180 SRNTF-----KVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLD- 233 R +VR + + +F +GF V TP++ A F + LDL+ Sbjct 129 PRTNLIGAVARVRHTLAQALHRFFNEQGFFWVSTPLITASDTEGAGEMF--RVSTLDLEN 186 Query 234 -------------------MYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISV-RHNP 273 +L ++ +L + +++ FR E + RH Sbjct 187 LPRNDQGKVDFDKDFFGKESFLTVSGQLNGETYACA-LSKIYTFGPTFRAENSNTSRHLA 245 Query 274 EFTMMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAI 333 EF M+E +A+A+ D+ L E++ + + + +L + D K F + ++A+ Sbjct 246 EFWMLEPEVAFANLNDIAGLAEAMLKYVFKAVLEERA--------DDMKFFAERVDKDAV 297 Query 334 KKYRP--ETDMADLDNFDSAKAIAESIGIHVEKS--WGLGRIVTEIFEEVAEAHLIQPTF 389 + E D A +D D A I E+ G E WG+ + +E +AE H P Sbjct 298 SRLERFIEADFAQVDYTD-AVTILENCGRKFENPVYWGVD-LSSEHERYLAEEHFKAPVV 355 Query 390 ITEYPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRF--LDQVAAK 447 + YP ++ R + + + + + G E+ + +R LD+ + Sbjct 356 VKNYPKDIKAFYMRLNEDGKTVAAMDV------LAPGIGEIIGGSQREERLDVLDERMLE 409 Query 448 DAGDDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFP 499 + E ++ Y +G P +G G+G +R++ T +RDVI FP Sbjct 410 MGLNKEDYWW---YRDLRRYGTVPHSGFGLGFERLIAYVTGVQNVRDVIPFP 458 > WP_000003071.1 on WP_000138915.1 MULTISPECIES: malate synthase A [Enterobacteriaceae] Length=533 Score = 30.8 bits (68), Expect = 0.20, Method: Compositional matrix adjust. Identities = 22/60 (37%), Positives = 30/60 (50%), Gaps = 8/60 (13%) Query 11 AVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEELEALNIEVAV 70 A+ N+ L +R+ +L +REQ T+DQL A DG+ EE NI VAV Sbjct 375 AMAVFNDILGSRKNQLEVMREQDAPI--------TADQLLAPCDGERTEEGMRANIRVAV 426 > WP_000003071.1 on WP_001301303.1 MULTISPECIES: IMPACT family member YigZ [Enterobacteriaceae] Length=204 Score = 28.1 bits (61), Expect = 1.0, Method: Compositional matrix adjust. Identities = 15/36 (42%), Positives = 21/36 (58%), Gaps = 0/36 (0%) Query 323 PFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESI 358 P +T+ E IKK R T +A D ++AKA ES+ Sbjct 7 PAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESV 42 > WP_000003071.1 on WP_000187022.1 MULTISPECIES: tRNA (uridine(54)-C5)-methyltransferase TrmA [Proteobacteria] Length=366 Score = 28.1 bits (61), Expect = 1.6, Method: Compositional matrix adjust. Identities = 24/96 (25%), Positives = 40/96 (42%), Gaps = 11/96 (11%) Query 50 HAEFDGKENEELEAL-------NIEVAVAGRMMTRRIMGKASFVTLQ-DVGGRIQLYVAR 101 H + D + +E EAL N+ V + GR +I ++ + V G+ +Y Sbjct 125 HKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIELDQDYIDERLPVAGKEMIYRQV 184 Query 102 DD---LPEGVYNEQFKKWDLGDILGAKGKLFKTKTG 134 ++ P N Q +W L G+KG L + G Sbjct 185 ENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCG 220 > WP_000003071.1 on WP_000263098.1 MULTISPECIES: DNA-directed RNA polymerase subunit beta [Proteobacteria] Length=1342 Score = 27.7 bits (60), Expect = 2.6, Method: Compositional matrix adjust. Identities = 19/62 (31%), Positives = 32/62 (52%), Gaps = 7/62 (11%) Query 203 FMEVETPMMQVIPGGAAARPFITHHNA----LDLDMYLRIAPELYLKRLVVG-GFERVFE 257 +M+V T QV+ GA+ PF+ H +A + +M + P L + +VG G ER Sbjct 652 YMDVST--QQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVA 709 Query 258 IN 259 ++ Sbjct 710 VD 711 Lambda K H a alpha 0.320 0.138 0.397 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 387975132 Database: Mentha_longifoli.pep Posted date: Jan 8, 2019 3:07 AM Number of letters in database: 1,311,348 Number of sequences in database: 4,329 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40