BLAST Results
TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Calotropis_gigantea.cds 20,832 sequences; 28,036,827 total letters Query= OUZ99072.1 Protein of unknown function DUF3754 [Macleaya cordata] Length=740 Score E Sequences producing significant alignments: (Bits) Value cal_g015653.t1 669 0.0 cal_g015653.t2 666 0.0 cal_g010355.t1 32.7 0.68 cal_g014477.t1 30.0 6.0 cal_g008746.t2 29.6 8.1 > OUZ99072.1 on cal_g015653.t1 Length=2154 Score = 669 bits (1726), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/661 (57%), Positives = 481/661 (73%), Gaps = 22/661 (3%) Frame = +1 Query 88 GGGIRDEK----GISGIRVPRQRYIFVSKKellesllsmf-esqqqIDDFLQLSSCLDSI 142 GG + D + ISGI+V R RYI VSK LL++++S E+Q + D F+QLS LD+I Sbjct 202 GGDLEDGEESSGTISGIQVARPRYISVSKSNLLKAIVSSMFETQGKADQFIQLSKYLDTI 381 Query 143 IHAEHKSILEEMRIDYSQTDSVEKQDFDSEDLQSTeeeeeeeeIVVNGKHSEKLDFDTEN 202 +HAEHKSILEEMRI+Y T VE + +D +E E+ +G K ++E+ Sbjct 382 LHAEHKSILEEMRINYDITQIVESKGIKHDDSPDSESMLVSSEMESDGDI--KKFVESES 555 Query 203 LQeedeeekddeRPLNFNYgldlsfllgssfKKTDVNSGAESRIAVATRFQRAFMKLLHN 262 E++ + ++P N G+ + +T+ GA +V T FQ AF++LLHN Sbjct 556 TVEDEARRAEQDKP-NCISGVHPQLCSSFNNDETNSIKGA----SVYTCFQHAFLQLLHN 720 Query 263 AQFEELSARDLLLTSALNTDYLLTLPIYVDWKRALESNAIIFRRGYATERQNGLLLVEKL 322 A+FEELSARDL LTSAL TDYLLTLPI +DWK A +SNAII+RRGY TE+Q GLL VEKL Sbjct 721 AEFEELSARDLQLTSALTTDYLLTLPINIDWKMASQSNAIIYRRGYTTEKQKGLLTVEKL 900 Query 323 DYLQSKLLQGIFFTLAKPVGRVGMYINEALKSASQTKEAQVWIERVKHWFKEPSLSQQSY 382 DYLQSKLLQ IF +AKP+G+ G+++ E L ++ +W ERVK W ++ SLSQQSY Sbjct 901 DYLQSKLLQRIFLLMAKPLGKFGIWLMEVLG-----RKKLLWAERVKLWLEKLSLSQQSY 1065 Query 383 SIHD-TFNDQLVADQLSDSDLPIWLAAQRAVPRYEGLLSSAGPRGRLLRKLLTWIGVIPS 441 S + T +++ +AD ++S+LPIWLAAQRAV RYEG+LS +GPR RL RKLL WIG+ PS Sbjct 1066 SCDEVTSSNEAIADHSTESELPIWLAAQRAVTRYEGILSGSGPRERLFRKLLVWIGLSPS 1245 Query 442 VPEASFELDSVNTTS--ELYARPIFLSRISLGDLWRPATRERCGN-VWNMLKTAVSILFS 498 E+SF+L S + S E RPIFL R+SL D+W PA+ E CGN +W + T +SIL S Sbjct 1246 AQESSFDLKSNTSGSHAESLLRPIFLPRVSLSDIWEPASWESCGNDLWKIFTTVISILLS 1425 Query 499 QSVLQEPAFQELILLYTEEIGQRESRDKIEVPSLKLKIYERIPIPDLPVIFPHKKLSFRI 558 QS LQEPAF+ELILLYT ++ + DK+++PSL+LKIYE+IPIPDLPVIFPHKKLSFRI Sbjct 1426 QSTLQEPAFKELILLYTRDLDGTNTTDKLKIPSLQLKIYEKIPIPDLPVIFPHKKLSFRI 1605 Query 559 LDTVRLDVASIVGLLAFLVNYKFENIVSSPSAFLLDAIAFSALVIYVSRVALGYKQTWDR 618 LDTVRLDVA+++GLLA+ +NYKFE+I SSPSA +LD IA +AL+IYV RV LGYKQT DR Sbjct 1606 LDTVRLDVATVLGLLAYFINYKFESISSSPSAIILDIIAVTALIIYVIRVVLGYKQTRDR 1785 Query 619 YQLLVNRTLYEKTLASGFGSVHFLLDASEQQQYKEAILAYAVLLQAENRQMTCHKSIGDA 678 YQLLVNRTL EKT+ASGFGSVHFLLDASEQQ +KEAILAYAVLL+ + +MT +S+GD Sbjct 1786 YQLLVNRTLSEKTVASGFGSVHFLLDASEQQLFKEAILAYAVLLRGQGDKMTA-RSVGDE 1962 Query 679 CERFLYDKLKEKVEMPVDNAMDTLLRLGLVTEASINGNISLRAIPCSEAYVVLKQRWNSL 738 CE F+ +K+EMP+D A TL RLGLV E +++G L+A+PCS+AY +L++RWN L Sbjct 1963 CEYFISSVFNKKIEMPIDKAKTTLSRLGLVIEKAVDGEAVLQAVPCSQAYEILRERWNGL 2142 Query 739 L 739 L Sbjct 2143 L 2145 > OUZ99072.1 on cal_g015653.t2 Length=2280 Score = 666 bits (1718), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/705 (54%), Positives = 483/705 (69%), Gaps = 68/705 (10%) Frame = +1 Query 88 GGGIRDEK----GISGIRVPRQRYIFVSKKellesllsmf-esqqqIDDFLQLSSCLDSI 142 GG + D + ISGI+V R RYI VSK LL++++S E+Q + D F+QLS LD+I Sbjct 202 GGDLEDGEESSGTISGIQVARPRYISVSKSNLLKAIVSSMFETQGKADQFIQLSKYLDTI 381 Query 143 IHAEHKSILEEMRIDYSQTDSVEKQDFDSEDLQSTeeeeeeeeIVVNGKHSEKLDFDTEN 202 +HAEHKSILEEMRI+Y T VE + +D +E E+ +G K ++E+ Sbjct 382 LHAEHKSILEEMRINYDITQIVESKGIKHDDSPDSESMLVSSEMESDGDI--KKFVESES 555 Query 203 LQeedeeekddeRPLNFNYgldlsfllgssfKKTDVNS--GAESRIAVATRFQRAFMKLL 260 E++ + ++P N + L SSF + NS GA +V T FQ AF++LL Sbjct 556 TVEDEARRAEQDKP---NCISGVHPQLCSSFNNDETNSIKGA----SVYTCFQHAFLQLL 714 Query 261 HNAQFEELSARDLLLTSALNTDYLLTLPIYVDWKRALESNAIIFRRGYATERQNGLLLVE 320 HNA+FEELSARDL LTSAL TDYLLTLPI +DWK A +SNAII+RRGY TE+Q GLL VE Sbjct 715 HNAEFEELSARDLQLTSALTTDYLLTLPINIDWKMASQSNAIIYRRGYTTEKQKGLLTVE 894 Query 321 KLDYLQSKLLQGIFFTLAKPVGRVGMYINEALKSASQTKEAQVWIERVKHWFKEPSLSQQ 380 KLDYLQSKLLQ IF +AKP+G+ G+++ E L ++ +W ERVK W ++ SLSQQ Sbjct 895 KLDYLQSKLLQRIFLLMAKPLGKFGIWLMEVLG-----RKKLLWAERVKLWLEKLSLSQQ 1059 Query 381 SYSIHD-TFNDQLVADQLSDSDLPIWLAAQRAVPRYEGLLSSAGPRGRLLRKLLTWIGVI 439 SYS + T +++ +AD ++S+LPIWLAAQRAV RYEG+LS +GPR RL RKLL WIG+ Sbjct 1060 SYSCDEVTSSNEAIADHSTESELPIWLAAQRAVTRYEGILSGSGPRERLFRKLLVWIGLS 1239 Query 440 PSVPEASFELDSVNTTS--ELYARPIFLSRISLGDLWRPATRERCGN-VWNMLKTAVSIL 496 PS E+SF+L S + S E RPIFL R+SL D+W PA+ E CGN +W + T +SIL Sbjct 1240 PSAQESSFDLKSNTSGSHAESLLRPIFLPRVSLSDIWEPASWESCGNDLWKIFTTVISIL 1419 Query 497 FSQSVLQEPAFQELILLYTEEIGQRESRDKIEVPSLKLKIYERIPIPDLPVIFPHKKLSF 556 SQS LQEPAF+ELILLYT ++ + DK+++PSL+LKIYE+IPIPDLPVIFPHKKLSF Sbjct 1420 LSQSTLQEPAFKELILLYTRDLDGTNTTDKLKIPSLQLKIYEKIPIPDLPVIFPHKKLSF 1599 Query 557 RILDTVRLDVASIVGLLAFLVNYKFENIVSSP---------------------------- 588 RILDTVRLDVA+++GLLA+ +NYKFE+I SSP Sbjct 1600 RILDTVRLDVATVLGLLAYFINYKFESISSSPGHGLQMSYKMVTEPIIAKVVKCMASQQG 1779 Query 589 --------------SAFLLDAIAFSALVIYVSRVALGYKQTWDRYQLLVNRTLYEKTLAS 634 SA +LD IA +AL+IYV RV LGYKQT DRYQLLVNRTL EKT+AS Sbjct 1780 KFLRRLSSRDISDQSAIILDIIAVTALIIYVIRVVLGYKQTRDRYQLLVNRTLSEKTVAS 1959 Query 635 GFGSVHFLLDASEQQQYKEAILAYAVLLQAENRQMTCHKSIGDACERFLYDKLKEKVEMP 694 GFGSVHFLLDASEQQ +KEAILAYAVLL+ + +MT +S+GD CE F+ +K+EMP Sbjct 1960 GFGSVHFLLDASEQQLFKEAILAYAVLLRGQGDKMTA-RSVGDECEYFISSVFNKKIEMP 2136 Query 695 VDNAMDTLLRLGLVTEASINGNISLRAIPCSEAYVVLKQRWNSLL 739 +D A TL RLGLV E +++G L+A+PCS+AY +L++RWN LL Sbjct 2137 IDKAKTTLSRLGLVIEKAVDGEAVLQAVPCSQAYEILRERWNGLL 2271 > OUZ99072.1 on cal_g010355.t1 Length=1473 Score = 32.7 bits (73), Expect = 0.68, Method: Compositional matrix adjust. Identities = 26/116 (22%), Positives = 49/116 (42%), Gaps = 32/116 (28%) Frame = +1 Query 283 YLLTLPIYVDWKRALESNA--------------------IIFRRGYATERQNGLLLVEKL 322 YLL LPI VD + L+ N +IFRRG +R +EK+ Sbjct 379 YLLNLPITVD-ESKLDKNLLKRYFAEHPKSDLPDFADKYVIFRRGIGIDRTTDFFFLEKV 555 Query 323 DYLQSKL---------LQGIFFTLAKPVGRVGMYINEALKSASQTKEAQVWIERVK 369 D + ++L L+ + + P + N+ + S+ ++ +++ER++ Sbjct 556 DMMIARLWAWLLRKTRLESLLSRRSNPRHKKNQMKNDGI--TSEAEQDDIYVERIR 717 > OUZ99072.1 on cal_g014477.t1 Length=5427 Score = 30.0 bits (66), Expect = 6.0, Method: Compositional matrix adjust. Identities = 14/32 (44%), Positives = 21/32 (66%), Gaps = 0/32 (0%) Frame = +3 Query 634 SGFGSVHFLLDASEQQQYKEAILAYAVLLQAE 665 +GFG VH+ LD+ ++YK L YA+L+ E Sbjct 1407 AGFGFVHYSLDSEFPREYKLEDLLYAILVVPE 1502 > OUZ99072.1 on cal_g008746.t2 Length=3234 Score = 29.6 bits (65), Expect = 8.1, Method: Compositional matrix adjust. Identities = 30/110 (27%), Positives = 47/110 (43%), Gaps = 18/110 (16%) Frame = -3 Query 500 SVLQEPAFQELILLYTEEIGQRESRDKIEVPSLKLKIYERIPIPDLPVIFP--------- 550 S Q P Q L+ + + S +P+L L+++ +P+ +P IF Sbjct 337 SEFQAPLQQNLLSYQILSLADQRSVYTFHLPALLLQVFSLLPV-KVPCIFALQTNHVQNW 161 Query 551 HKKLSFRILDTVRLDVASIVGLLAFLVNYKFENIVSSPSAFLLDAIAFSA 600 K S L VR+ L F++ Y F N+V+ +AF L FSA Sbjct 160 SKNNSVSHLREVRM--------LRFVMRYSFYNLVA*QNAFSLKMDIFSA 35 Lambda K H a alpha 0.320 0.135 0.387 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 4545488115 Database: Calotropis_gigantea.cds Posted date: Jan 8, 2019 1:24 AM Number of letters in database: 28,036,827 Number of sequences in database: 20,832 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40