BLAST Results
BLASTP 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Calotropis_gigantea.pep 20,832 sequences; 9,345,609 total letters Query= OUZ99070.1 Plastid lipid-associated protein/fibrillin conserved domain [Macleaya cordata] Length=302 Score E Sequences producing significant alignments: (Bits) Value cal_g015654.t1 348 4e-119 cal_g015654.t2 219 1e-69 cal_g002960.t1 45.4 1e-05 cal_g005289.t2 43.5 7e-05 cal_g005289.t1 43.5 7e-05 cal_g006416.t1 38.9 0.001 cal_g010684.t1 37.0 0.007 cal_g014133.t1 32.0 0.23 > OUZ99070.1 on cal_g015654.t1 Length=311 Score = 348 bits (893), Expect = 4e-119, Method: Compositional matrix adjust. Identities = 166/238 (70%), Positives = 199/238 (84%), Gaps = 5/238 (2%) Query 63 VRCLATTANSRAIDEFELESKKHDLLRAIQDTQRGLLTTPDQRSSIEEALVCVEGYDAGT 122 VRC A ++ ++E +K LLR IQ+TQRGL+TT +QRSSIEEALV VEG DAG Sbjct 77 VRCSAFRGVTKQVEE-----EKCQLLRVIQNTQRGLVTTTEQRSSIEEALVSVEGLDAGD 131 Query 123 AIDLGKLDGTWRLQYTSAPDVVVLFESTQRFPFFQVGQIFQKFECRDQTDGGVVRNIVQW 182 +DL +L+GTWRLQYTSAPDV++L ES R PFFQ+GQIFQKFEC +++GGV+RNIV+W Sbjct 132 GVDLAELNGTWRLQYTSAPDVLILLESATRLPFFQIGQIFQKFECGGESNGGVLRNIVKW 191 Query 183 SIPTLLEEQEGATLLVSAKFSVVSKRNIYLQFEEVSVQNIKISEELQSLIAPALLPRTYL 242 SIP +LEEQEGATLLVSAKFS +S R+IYLQFEE+ VQNI IS+ +Q+LIAPALLPR++L Sbjct 192 SIPNILEEQEGATLLVSAKFSFISSRSIYLQFEEIGVQNIIISDYVQALIAPALLPRSFL 251 Query 243 SLQILQFIQTFKAAVPVRSPGRQSVGGLYYLSFLDNNMLLGRAVGGGGVFVFTKAQPF 300 SLQ+LQFIQ+FKA +PV S R SVGGLYYLS+LD NMLLGRAVGGGGVFVFT+AQP Sbjct 252 SLQVLQFIQSFKAQIPVTSQSRNSVGGLYYLSYLDRNMLLGRAVGGGGVFVFTRAQPL 309 > OUZ99070.1 on cal_g015654.t2 Length=230 Score = 219 bits (557), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 104/155 (67%), Positives = 126/155 (81%), Gaps = 5/155 (3%) Query 63 VRCLATTANSRAIDEFELESKKHDLLRAIQDTQRGLLTTPDQRSSIEEALVCVEGYDAGT 122 VRC A ++ ++E +K LLR IQ+TQRGL+TT +QRSSIEEALV VEG DAG Sbjct 77 VRCSAFRGVTKQVEE-----EKCQLLRVIQNTQRGLVTTTEQRSSIEEALVSVEGLDAGD 131 Query 123 AIDLGKLDGTWRLQYTSAPDVVVLFESTQRFPFFQVGQIFQKFECRDQTDGGVVRNIVQW 182 +DL +L+GTWRLQYTSAPDV++L ES R PFFQ+GQIFQKFEC +++GGV+RNIV+W Sbjct 132 GVDLAELNGTWRLQYTSAPDVLILLESATRLPFFQIGQIFQKFECGGESNGGVLRNIVKW 191 Query 183 SIPTLLEEQEGATLLVSAKFSVVSKRNIYLQFEEV 217 SIP +LEEQEGATLLVSAKFS +S R+IYLQFEEV Sbjct 192 SIPNILEEQEGATLLVSAKFSFISSRSIYLQFEEV 226 > OUZ99070.1 on cal_g002960.t1 Length=323 Score = 45.4 bits (106), Expect = 1e-05, Method: Compositional matrix adjust. Identities = 62/236 (26%), Positives = 102/236 (43%), Gaps = 30/236 (13%) Query 84 KHDLLRAIQDTQRGLLTTPDQRSSIEEALVCVEGYDAGTAID--LGKLDGTWRLQYTSAP 141 K L+ + T GL + + R+ + E + +E + L L+G W L YTS Sbjct 99 KKQLVDSFYGTNLGLTASSETRAEVVELITQLEAKNPTPVPTEALSLLNGKWILAYTS-- 156 Query 142 DVVVLFESTQR--FPFFQVGQIFQKFECRDQTDGGVVRNIVQWSIPTLLEEQEGATLLVS 199 V LF R P +V +I QK + + T V N VQ++ P ++ + Sbjct 157 -FVGLFPLLSRGTIPLVRVEEISQKIDSENFT----VENSVQFAGPLAT-----TSISTN 206 Query 200 AKFSVVSKRNIYLQFEEVSV------QNIKISEELQSL-----IAPALLPRTYLSLQILQ 248 AKF V S + + L+FEE + +I++ E +Q L +AP T L Sbjct 207 AKFEVRSPKRVQLKFEEGIIGTPQLTDSIELPENVQFLGQNIDLAPVKGLFTSLQNTASS 266 Query 249 FIQTFKAAVPVRSPGRQSVGGLYYL-SFLDNNMLLGRAVGGGGVFVFTK-AQPFLS 302 ++ + P++ S + L ++LDN + + R GG +FV K P L+ Sbjct 267 VARSISSQPPLKFSISSSNAESWLLTTYLDNELRISRG-DGGSIFVLIKEGSPLLT 321 > OUZ99070.1 on cal_g005289.t2 Length=387 Score = 43.5 bits (101), Expect = 7e-05, Method: Compositional matrix adjust. Identities = 67/246 (27%), Positives = 101/246 (41%), Gaps = 40/246 (16%) Query 74 AIDEFELESKKHDLLRAIQDTQRGLLTTPDQRSSIE---EALVCVEGYDAGTAIDLGK-- 128 A D+ K DL RA DT G T R+S E EA+ + +A Sbjct 150 AGDDVGESEKLRDLKRAFVDTVYG--TDFGLRASTEVRAEAIELISQLEAANPTPAPTET 207 Query 129 ---LDGTWRLQYTSAPDVVVLFESTQRFPFFQVGQIFQKFECRDQTDGGVVRNIVQWSIP 185 LDG W L YT+ +++ L + P +V +I Q + T V N + S P Sbjct 208 PELLDGNWVLVYTAFSELLPLL-AVGNLPLVKVDRICQSIDSSSLT----VDNSITISGP 262 Query 186 TLLEEQEGATL--LVSAKFSVVSKRNIYLQFEEVSVQ------NIKISEELQSLIAPALL 237 ATL SA F V S I +QF+E +++ NI + E++ L Sbjct 263 -------AATLSFSASAAFEVRSPTRILVQFKEGTLKPPEIKSNIDLPEDVNVFGQTISL 315 Query 238 PRTYLSLQILQ-----FIQTFKAAVPVR--SPGRQSVGGLYYLSFLDNNMLLGRAVGGGG 290 SL LQ +T P++ PG ++ L +++LD + + R G GG Sbjct 316 SPVQQSLDPLQNAVAGIARTISGQPPLKIPIPGERAASWL-LITYLDKDFRISR--GDGG 372 Query 291 VFVFTK 296 +FV + Sbjct 373 LFVLAR 378 > OUZ99070.1 on cal_g005289.t1 Length=387 Score = 43.5 bits (101), Expect = 7e-05, Method: Compositional matrix adjust. Identities = 67/246 (27%), Positives = 101/246 (41%), Gaps = 40/246 (16%) Query 74 AIDEFELESKKHDLLRAIQDTQRGLLTTPDQRSSIE---EALVCVEGYDAGTAIDLGK-- 128 A D+ K DL RA DT G T R+S E EA+ + +A Sbjct 150 AGDDVGESEKLRDLKRAFVDTVYG--TDFGLRASTEVRAEAIELISQLEAANPTPAPTET 207 Query 129 ---LDGTWRLQYTSAPDVVVLFESTQRFPFFQVGQIFQKFECRDQTDGGVVRNIVQWSIP 185 LDG W L YT+ +++ L + P +V +I Q + T V N + S P Sbjct 208 PELLDGNWVLVYTAFSELLPLL-AVGNLPLVKVDRICQSIDSSSLT----VDNSITISGP 262 Query 186 TLLEEQEGATL--LVSAKFSVVSKRNIYLQFEEVSVQ------NIKISEELQSLIAPALL 237 ATL SA F V S I +QF+E +++ NI + E++ L Sbjct 263 -------AATLSFSASAAFEVRSPTRILVQFKEGTLKPPEIKSNIDLPEDVNVFGQTISL 315 Query 238 PRTYLSLQILQ-----FIQTFKAAVPVR--SPGRQSVGGLYYLSFLDNNMLLGRAVGGGG 290 SL LQ +T P++ PG ++ L +++LD + + R G GG Sbjct 316 SPVQQSLDPLQNAVAGIARTISGQPPLKIPIPGERAASWL-LITYLDKDFRISR--GDGG 372 Query 291 VFVFTK 296 +FV + Sbjct 373 LFVLAR 378 > OUZ99070.1 on cal_g006416.t1 Length=222 Score = 38.9 bits (89), Expect = 0.001, Method: Compositional matrix adjust. Identities = 35/136 (26%), Positives = 57/136 (42%), Gaps = 17/136 (13%) Query 45 NTNLPSRHHHHHHHHRSTVRCLATTANSRAIDEFELESKKHDLLRAIQDTQRGLLT--TP 102 ++ P RH + L T + S D+ + S K LL I D RGL T P Sbjct 20 KSSFPRRHCQN----------LLTPSRSSLTDQSQTLSAKSHLLSLISDQDRGLKTQSDP 69 Query 103 DQRSSIEEALVCVEGYDAGTAIDLGKLDGTWRLQYTSAPDVVVLFESTQRFPFF--QVGQ 160 + + I +A+ V T L GTWRL +T+ + + + ++ P F + G Sbjct 70 QKLAQIIQAVDAVADVGRDTVTTESLLSGTWRLLWTTEKEQLYIIKNA---PLFGTKAGD 126 Query 161 IFQKFECRDQTDGGVV 176 + Q + +T V+ Sbjct 127 VLQVIDVDKKTLNNVI 142 > OUZ99070.1 on cal_g010684.t1 Length=259 Score = 37.0 bits (84), Expect = 0.007, Method: Compositional matrix adjust. Identities = 47/255 (18%), Positives = 92/255 (36%), Gaps = 64/255 (25%) Query 54 HHHHHHRSTVRCLATTANSRAIDEF--------ELESKKHDLLRAIQDTQRGLLTTPDQR 105 HHHH + + C ++ + F + + K LL AI+ RG TP+ + Sbjct 53 HHHHRYGFSTSCSSSQKWRTRVSFFPAFLNNKKDANAIKEKLLEAIEPLDRGAEATPEDQ 112 Query 106 SSIEEALVCVEGYD-AGTAIDLGKLDGTWRLQYTSAPDVVVLFESTQRFPFFQVGQIFQK 164 ++E +E + + LDG W L YT++ ++ T+R F + +Q Sbjct 113 KRVDEIARKLEAANPTREPLKSDLLDGKWELIYTTSQSIL----QTKRPKFLRSRTNYQA 168 Query 165 FECRDQTDGGVVRNIVQWSIPTLLEEQEGATLLVSAKFSVVSKRNIYLQFEEVSVQNIKI 224 D +N+ W P + V+A + ++ R + ++F+ + + Sbjct 169 INV----DTLRAQNMESW--PFFNQ--------VTADLTPLNTRKVAVKFDYFKIGGL-- 212 Query 225 SEELQSLIAPALLPRTYLSLQILQFIQTFKAAVPVRSPGRQSVGGLYYLSFLDNNMLLGR 284 +PV++P R G +++LD + + R Sbjct 213 --------------------------------IPVKAPER--ARGELEITYLDEELRISR 238 Query 285 AVGGGGVFVFTKAQP 299 G +F+ P Sbjct 239 G-DKGNLFILKMVDP 252 > OUZ99070.1 on cal_g014133.t1 Length=246 Score = 32.0 bits (71), Expect = 0.23, Method: Compositional matrix adjust. Identities = 21/63 (33%), Positives = 29/63 (46%), Gaps = 2/63 (3%) Query 80 LESKKHDLLRAIQDTQRGLLTTPDQRSSIEEALVCVEGYDAGTAI--DLGKLDGTWRLQY 137 + S K LL A+ RGL + D + A +E + DL KL G W+L Y Sbjct 86 IASLKFKLLSAVSGLNRGLAASEDDLQKADNAAKDLESVSGLVDLSDDLDKLQGRWKLIY 145 Query 138 TSA 140 +SA Sbjct 146 SSA 148 Lambda K H a alpha 0.320 0.135 0.398 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 1501605525 Database: Calotropis_gigantea.pep Posted date: Jan 8, 2019 1:06 AM Number of letters in database: 9,345,609 Number of sequences in database: 20,832 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40